Fig 1: CD226+ Cells Were Mainly Activated Effector CD4+ or CD8+ T Cells(A) In the biopsies of patients with DM (n = 5) and IMNM (n = 3), CD226+ cells were strong coexpressed CD4 or CD8 cells. (B) A representative staining from a patient with DM showed that most of the CD226+ cells were CD226+CD69+ or CD226+HLA-DR+ phenotypes. (C) In PBMCs isolated from patients with DM, the production percentages of IFN-? and TNF-a of CD4+CD226+ T cells and CD107a expression percentages of CD8+CD226+ T cells were significantly higher than those of the CD226-negative counterparts in patients with DM (n = 5). (D) Costaining of CD226 and CD244 in the DM sections (n = 3) showed that the CD226+ cells did not colocalize with CD244+ cells. (A, B, and D, original magnification ×400). *p < 0.05, **p < 0.01, ***p < 0.001. DM = dermatomyositis; IFN-? = intracellular interferon-?; IMNM = immune-mediated necrotizing myopathy; TNF-a = tumor necrosis factor a.