Fig 1: miR-708 and NF-?B inhibition decreases IKKß and COX-2 protein expression, respectively, in TNFa/IL-1ß-treated diseased lung cells. (a) Representative western blot examining COX-2 and IKKß (IKK-b) protein expression in A549 cells transiently transfected with mock (left) or 25 nM miR-708 (right) in combination with TNFa/IL-1ß for 0–48 hours. (b) Representative western blot examining COX-2 and IKKß (IKK-b) protein expression in A549 cells transiently transfected with mock (left), 25 nM miR-708 (middle), or 10 µM BAY-11-7082 in combination with TNFa/IL-1ß for 0–24 hours. (c) Quantification of IKKß protein expression from representative western blot (b) and others (data not shown). IKKß expression was normalized to GAPDH protein expression and TNFa/IL-1ß 0 h (left black bar). *p < 0.05 and **p < 0.01, n = 3.