Fig 1: The standard calibration curve generated using the AlphaLISA with solutions containing recombinant ST2 at selected concentrations.
Fig 2: ST2 receptor contributes to IL-33-induced gastric cancer cell invasion and migration. (A and B) Cell invasion of AGS and MKN45 cells was detected by Transwell assays (magnification, ×200). (C and D) Cell migration of AGS and MKN45 cells was detected by wound healing assays (magnification, ×100). One-way ANOVA was used for comparison among multiple groups. All experiments were performed in triplicate. *P<0.05 IL-33 vs. NC; #P<0.05 IL-33+siST2 vs. IL-33. NC, negative control; si-, small interfering RNA.
Fig 3: IL-33 induces activation of ERK1/2, JNK and p38 via ST2 receptor. (A-C) The protein expression and phosphorylation levels of ERK1/2, JNK and p38 were detected by western blotting. One-way ANOVA was used for comparison among multiple groups. All experiments were performed in triplicate. *P<0.05 IL-33 vs. NC; #P<0.05 IL-33+siST2 vs. IL-33. NC, negative control; si-, small interfering RNA; p-, phosphorylated.
Fig 4: IL-33/ST2 axis promotes gastric cancer cell cycle progression. Cell cycle of (A) AGS and (B) MKN45 cells was detected by flow cytometry. (C) The protein expression of cell cycle-related proteins CDK4, CDK6 and Cyclin D1 were detected by western blotting. One-way ANOVA was used for comparison among multiple groups. All experiments were performed in triplicate. *P<0.05 IL-33 vs. NC; #P<0.05 IL-33+siST2 vs. IL-33. GC, gastric cancer; NC, negative control; si-, small interfering RNA.
Fig 5: ST2 is highly expressed in GC tissues and IL-33/ST2 axis promotes proliferation of GC cells. (A) ST2 expression in GC tissues and adjacent normal tissues was examined by immunohistochemical analysis. Top panel magnification, ×100; bottom panel magnification, ×400. (B) mRNA and (C-E) protein expression levels of ST2 in AGS and MKN45 cells were detected by reverse transcription-quantitative PCR and western blotting. (F) Cell proliferation of AGS and MKN45 cells was measured using Cell Counting Kit-8 assays. Student's t-test was used for comparison between two groups, and one-way ANOVA was used for comparison among multiple groups. All experiments were performed in triplicate. *P<0.05 IL-33 vs. NC; #P<0.05 IL-33+siST2 vs. IL-33. GC, gastric cancer; NC, negative control; si-, small interfering RNA.
Supplier Page from Abcam for Anti-ST2 antibody