Fig 1: Predicted structure and sequence alignments of EgGps. (A) Modeled structure of EgGp1. (B) Sequence alignments of EgGps and corresponding amino sequences from other species. “*” symbols denote the positions of amino acids that have a single fully conserved amino acid residue; “:” symbols denote conservation between groups of amino acids with strongly similar properties; “.” symbols denote conservation between groups of amino acids with weakly similar properties; and “–” symbols denote gaps inserted to maximize sequence alignment. Amino acid side chains that participate in subunit contact or ligand binding are labeled as follows: dimer contact (green background); p, Ser phosphorylation site; h, G6P binding site; a, nucleotide activator site (AMP binding site); v, PLP- binding residue; g, active site residues (glucose binding); s, glycogen storage site; c, purine inhibitor (caffeine) site; EgGp1, Echinococcus granulosus glycogen phosphorylase 1 (MN562583); EgGp2, E. granulosus glycogen phosphorylase 2 (MN562584); HmGP, Hymenolepis microstoma glycogen phosphorylase (NP_002854.3); PYGL, Homo sapiens glycogen phosphorylase, liver form (NP_002854.3); PYGB, Homo sapiens glycogen phosphorylase, brain form (NP_002853.2); and PYGM, Homo sapiens glycogen phosphorylase, muscle form (NP_005600.1). (C) Analysis of compound docking to the target proteins; the red dashes represent H-bonds.
Fig 2: Relative mRNA expression levels (?Ct values) and localization of EgGps, and effects of compounds on EgGps. (A) Relative mRNA expression levels of the target genes, ?Ct = (Ct of target genes) – (Ct of EF-1a). PSCs, protoscoleces. *P < 0.01. (B) Gps in Echinococcus granulosus PSC lysates (20 µg of total protein); lane 1: anti-PYGM, lane 2: anti-PYGL, lane 3: anti-PYGB. (C) Localization of Gps in E. granulosus PSCs (confocal microscopy, anti-PYGB antibody). The anti-PYGM antibody failed to label proteins and the anti-PYGL yielded results similar to those yielded by the anti-PYGB antibody. (D) The effects of mefloquine (MEF), JF16, and ursolic acid (UA) on EgGps in vitro. (E) The effects of MEF, JF16, and UA on Rabbit muscle Gps in vitro.
Supplier Page from Abcam for Anti-PYGM antibody