Fig 1: Expression of PRR.Representative IHC stained images of PRR (brown), including 2 LGCA samples (A, C) and their patient-matched NC tissues (B, D), and 2 HGCA samples (E, G) and their patient-matched NC tissues (F, H). Nuclei were counterstained with hematoxylin (A-H, blue); original magnification: 400x. PRR mRNA expression levels were measured by RT-qPCR in 6 LGCA and 6 HGCA tissues (I) and in 3 LGCA and 3 HGCA primary cell lines that were sorted into EpCAMHigh (+) and EpCAMLow (-) subpopulations (J). Abundance in CA tissues is displayed relative to patient-matched NC tissues, and relative to a pool of NC tissues for the CA-derived cell lines, with error bars representing standard deviation. PRR protein expression by cell lines was detected by WB (K; 35 kDa); positive control = tonsil. Adapted from [38] under a CC BY license, with permission from Munro MJ et al. 2021.
Fig 2: Expression of AT2R.Representative IHC stained images of AT2R (brown), including 2 LGCA samples (A, C) and their patient-matched NC tissues (B, D), and 2 HGCA samples (E, G) and their patient-matched NC tissues (F, H). Nuclei were counterstained with hematoxylin (A-H, blue); original magnification: 400x. AT2R mRNA expression levels were measured by RT-qPCR in 6 LGCA and 6 HGCA tissues (I) and in 3 LGCA and 3 HGCA primary cell lines that were sorted into EpCAMHigh (+) and EpCAMLow (-) subpopulations (J). Abundance in CA tissues is displayed relative to patient-matched NC tissues, and relative to a pool of NC tissues for the CA-derived cell lines, with error bars representing standard deviation. PRR protein expression by cell lines was detected by WB (K; 45 kDa); positive control = HepG2 cells. Adapted from [38]. under a CC BY license, with permission from Munro MJ et al. 2021.
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