Fig 1: HDAC6 contributed to basal autophagy in AGE-stimulated podocytes. The activity of HDAC6 was assessed in podocytes treated with si-HDAC6 (A) and pc-HDAC6 (B), (A): n = 4, 4 independent experiments; (B): n = 4, 4 independent experiments. Representative Western blot analyses of Nephrin (C), LC3-II, P62 and Beclin-1 and densitometric analysis were obtained in AGE-treated podocytes cultured with si-HDAC6, tubacin (D-E) or pc-HDAC6 (F-G), (C): n = 3, 3 independent experiments; (E): n = 4, 4 independent experiments; (G): n = 4 or 5, 4 or 5 independent experiments (5 for P62, 4 for the others). AGE, advanced glycation end products; BSA, bovine serum albumin; HDAC6, histone deacetylase 6; pc-HADC6, pcDNA3.1-HDAC6; si-HDAC6, siRNA of HDAC6