Fig 2: MVSCs differentiated into perivascular cells in regenerating nerve tissue. IHC analysis of the middle segment of the MVSC-transplanted conduits at 2-month post-transplantation was performed by staining for endothelial cell marker (CD31) and perivascular cell markers (α-SMA and CNN1). MVSCs were labeled by GFP. Red color indicates positive staining for individual marker; blue color indicates positive DAPI staining; green color indicates GFP+ cells (scale bars: 100 μm in the left column, 10 μm in the right column)

Fig 3: In vitro characterization and differentiation of MVSCs isolated from rat aorta. a MVSCs were isolated from the aorta of GFP rats by using a tissue explant culture method, expanded, and stained for Sox10, Sox17, and Ki67. b MVSCs were stained for vascular cell markers α-SMA, CNN1, and CD31. c MVSCs were differentiated into peripheral neurons (Peripherin+) and Schwann cells (S100β+) in vitro. Red color indicates positive staining for individual marker; blue color indicates positive DAPI staining. Scale bar, 50 μm