Fig 1: Relative expression of TRPV1, TRPV3 and TRPV4 in myometrial tissue.Representative images from histological staining of native uterine tissue sections using A: hematoxylin & eosin () and B: Masson’s trichrome stains. Immunohistochemistry staining of C: TRPV1, D: TRPV3 and E: TRPV4, compared to F: IgG isotype (negative control) of native uterine tissue sections. Scale bar represents 100 µm.
Fig 2: Immuno-cyto-fluorescence and Western Blot analyses in USMCs and myometrial tissue subcellular fractions.A: Fluoro-immunocytochemistry detection of TRPV protein isoforms in cell culture derived from human myometrial tissues, a: DAPI staining of the nuclei, b: Anti TRPV1, c: Anti TRPV3, d: Anti-TRPV4 revealed by a secondary antibody coupled to ALEXA fluor 488. B: Detection of three TRPV proteins in the cytosolic and microsomal fractions prepared from human myometrial tissues at term, from two independent patients (P1 and P2). From top to bottom: anti-TRPV1, anti-TRPV3, anti-TRPV4 and anti-ß-actin (loading control) immunoblots are shown. Collectively, these immunostainings of the different TRPV isoforms confirmed the presence of the ligand-gated ion channels in myometrial cells and microsomal fractions from five independent preparations of human myometrial tissue.
Fig 3: Western blot and RT-PCR showing TRPV3 overexpression and knockdown in Caco-2 (A) and HCT8 (B) cells. Analysis of cell proliferation (C,D) and migration (E,F; crystal violet staining, 200×) abilities of Caco-2 and HCT8 cells with or without TRPV3 overexpression/knockdown. **, P<0.01; ***, P<0.001. TRPV3, transient receptor potential vanilloid 3; NC, negative control; sh, short hairpin; oe, overexpression; OD, optical density; RT-PCR, reverse transcription polymerase chain reaction.
Fig 4: X-tile analysis of overall survival was performed using patient data from GSE39582 to determine the optimal cuto? values for TRPV3 expression. The optimal cuto? values highlighted by the black circles in left panel (A) are shown in histogram of the entire cohort (B), and Kaplan-Meier plot is displayed in the right panel (C). Immunohistochemical staining of CRC tumor tissues and paired normal tissue microarrays from patients at our cancer center (D). Kaplan-Meier survival analysis of the identified 100 patients with low- and high-TRPV3 expression (E). TRPV3, transient receptor potential vanilloid 3; CRC, colorectal cancer.
Fig 5: GSEA analysis based on patients with low- and high-TRPV3 expression from GSE39582 dataset (A). Enrichment plot of MAPK pathway (B). Phosphorylation state of p-p38, p-JNK, p-cJun, and p-MEK1 in Caco-2 (C,D) and HCT8 (E,F) cells with or without TRPV3 overexpression/knockdown. *, P<0.05; **, P<0.01; ***, P<0.001. KEGG, Kyoto Encyclopedia of Genes and Genomes; FDR, false discovery rate; MAPK, mitogen-activated protein kinase; TRPV3, transient receptor potential vanilloid 3; NC, negative control; sh, short hairpin; oe, overexpression; GSEA, Gene Set Enrichment Analysis.
Supplier Page from Abcam for Anti-TRPV3 antibody