Fig 1: CLEC4M overexpression inhibits the proliferation of HCC cells. The viability of infected (A) Huh7 and (B) PLC/PRF/5 cells was assessed using a Cell Counting Kit-8 assay. The proliferation of infected (C) Huh7 and (D) PLC/PRF/5 cells was evaluated with an EdU assay. Data are presented as the mean ± standard deviation. *P<0.05. CLEC4M, C-type lectin domain family 4 member M; HCC, hepatocellular carcinoma; EdU, 5-Ethynyl-2'-deoxyuridine; OD, optical density; Vector, negative control.
Fig 2: CLEC4M overexpression is associated with favorable prognosis. The association of CLEC4M expression with 5-year (A) OS, (B) RFS, (C) PFS and (D) DSS was determined by analyzing patients with hepatocellular carcinoma included in the KMplot™ database. CLEC4M, C-type lectin domain family 4 member M; OS, overall survival; RFS, relapse-free survival; PFS, progression-free survival; DSS, disease-specific survival; HR, hazard ratio.
Fig 3: CLEC4M overexpression induces apoptosis in HCC cells. Apoptosis in infected (A) Huh7 and (B) PLC/PRF/5 cells was assessed via flow cytometric assays. Data are presented as the mean ± standard deviation. *P<0.05. CLEC4M, C-type lectin domain family 4 member M; HCC, hepatocellular carcinoma; Vector, negative control; PI, propidium iodide; Q, quadrant.
Fig 4: CLEC4M overexpression in unpaired non-tumor liver tissue samples is compared with HCC tissue samples. CLEC4M mRNA expression levels in unpaired non-tumor liver and hepatocellular carcinoma tumor tissue samples were examined in the (A) GSE14520_GPL571, (B) GSE14520_GPL3921, (C) GSE14323_GPL571 and (D) GSE6764 datasets from the Oncomine™ database. *P<0.05. CLEC4M, C-type lectin domain family 4 member M; HCC, hepatocellular carcinoma.
Fig 5: CLEC4M overexpression inhibits the JAK1/STAT3 pathway. p-JAK1, JAK1, p-STAT3, STAT3 and GAPDH protein levels were assessed in infected (A) Huh7 and (B) PLC/PRF/5 via western blotting. Data are presented as the mean ± standard deviation. *P<0.05. CLEC4M, C-type lectin domain family 4 member M; p-, phosphorylated; JAK1, Janus kinase 1; STAT3, signal transducer and activator of transcription 3; Vector, negative control.
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