Fig 1: Identification of BMSCs and EVs. The BMSCs obtained from ATCC were subcultured. A) The morphology of BMSCs in the fourth generation was observed by microscope. B) The positive marker proteins (CD29, CD44, and CD71) were positively expressed and negative marker proteins (HLA-DR, CD34, and CD45) of BMSCs were negatively expressed detected using flow cytometry. C) Lipid differentiation was observed by oil red O staining. D) Alizarin red staining was used to observe osteogenic differentiation; the EVs were extracted. E) The morphology of BMSCs-EVs was observed with a typical cup shape by TEM. F) The size distribution of EVs was mainly at about 100 nm analyzed using NTA. G) WB detected that the expressions of EV surface CD9, CD63, and CD81 enrichment and calnexin protein were not significantly expressed.
Supplier Page from Abcam for Anti-CD44 antibody [IM7] (PE)