Fig 1: TK1 knockdown relieves apoptosis, cell cycle arrest and senescence in SLE BMSCs. (A–E) The expression of cell cycle- and apoptosis-relevant proteins was measured in SLE BMSCs with TK1 overexpression or TK1 knockdown through western blotting, containing (B) Cleaved caspase-3, (C) Bax, (D) Bcl-2 as well as (E) CyclinD1. (F,G) Cell cycle proportions were tested in specified SLE BMSCs via flow cytometry analyses. (H–K) The expression of senescence-relevant proteins was measured in specified SLE BMSCs. Ns: not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
Fig 2: Silencing TK1 relieves inflammatory response and apoptosis in SLE BMSCs. (A) TK1 mRNA expression was measured in SLE BMSCs with TK1 overexpression or TK1 knockdown through RT-qPCR. (B,C) TK1 protein expression was tested in SLE BMSCs with TK1 overexpression or TK1 knockdown via western blotting. (D) IL-1ß levels were quantified in the supernatant of specified SLE BMSCs through ELISA. (E) RT-qPCR was adopted to measure the mRNA expression of IL-1ß in specified SLE BMSCs. (F,G) Flow cytometry analyses were conducted to test the apoptosis levels of specified SLE BMSCs. **p < 0.01; ***p < 0.001; ****p < 0.0001.
Fig 3: Signaling pathways involving characteristic genes. (A–J) GSEA enrichment analyses of high and low expression of characteristic genes: (A,B) CXCL1, (C,D) CXCL2, (E,F) CXCL8, (G,H) CXCR1 and (I,J) TK1 in the GSE50772 dataset.
Fig 4: Activation of inflammatory response in SLE and up-regulation of TK1 in SLE BMSCs. (A–F) Serum levels of (A) IFN-?, (B) IL-12, (C) IL-6, (D) IL-13, (E) IL-18 as well as (F) IL-1ß were measured in twenty SLE patients and twenty controls through ELISA. (G–I) TK1 mRNA expression was tested in BMSCs from five SLE patients and five controls through (G) RT-qPCR and (H,I) western blotting. ***p < 0.001; ****p < 0.0001.
Fig 5: Analyses of diagnostic efficacy of the LASSO model and associations of characteristic genes with infiltrating immune cells. (A) ROCs for evaluating the diagnostic efficacy of the LASSO model both in the GSE50772 and GSE81622 datasets. (B) Heatmap depicts the associations of characteristic genes (CXCL1, CXCL2, CXCL8, CXCR1 and TK1) with infiltrating immune cells in the GSE50772 dataset. *p < 0.05; **p < 0.01.
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