Fig 1: Localization of differentially expressed proteins in stereological cell types. EEF1A1 (A and C) labels neurons as does the neuronal-specific antibody MAP2 (B and C). PLP1 (D and F) colocalizes with the axonal marker PAN (E and F). YWHAH (G and I) is especially localized in the nucleus of MAP2-tagged neurons (H and I). ORM2 (J and L) barely colocalizes with GFAP (K and L). Dashed lines delineate neuronal somas. Arrows point to colocalizations. Scale bar represents 20 μm.
Fig 2: Summary of proteomic results. The diagram shows the different bioinformatics analyses carried out, differentially expressed proteins (DEPs) obtained, and those that have passed the criteria established for their validation. A, Dia-PASEF proteomic analysis identified 2202 proteins, of which 2167 were quantified. One hundred ninety-nine differentially expressed proteins (94 upregulated and 105 downregulated) were obtained using a p-value <0.05 and a fold change ≥1.3 and ≤0.7. B, of the total proteins that are composed of the annotated interactome of the SNCA (562 proteins), 35.7% (201/562) were quantified in this study, while 12.43% of the quantified proteins that interact with SNCA are DEPs (25/201). C, 22.7% (493/2167) of the quantified proteins comprised synaptic cellular components (CC) and synaptic biological processes (BP), and 9.3% (46/493) of these were DEPs. D, five upregulated (red) DEPs (GNAI1, EEF1A1, PLP1, NPTN, YWHAY) and two downregulated (green) DEPs (GRIM19, ORM2) were selected for the study according to the criteria established for it. The few matching DEPs of the annotated interactome of the SNCA and comprised synaptic CC and synaptic BP did not meet the criteria for selection. dia-PASEF, data-independent acquisition-parallel accumulation serial fragmentation.
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