Fig 1: BHB treatment reduced demyelination in CPZ-fed mice. (A) Histological evaluation of demyelination by LFB/CV staining. Scale bars = 50 μm. (B) Representative MBP staining and intensity quantification in the corpus callosum and hippocampus of CPZ-fed mice among different groups. Scale bars = 50 μm. (C) Western blot analysis of NG2 expression in CPZ-fed mice. (D and E) Representative TEM images of demyelination in the corpus callosum of CPZ-fed mice. Note that non-myelinated axons (red asterisks) are frequent in CPZ-fed mice; D magnified 4,000×, scale bars = 2 μm. E magnified 10,000×, scale bars = 1 μm. Data are presented as mean ± SEM. N = 3 per experimental group; experiment repeated two times. **p < 0.01 and ***p < 0.001 versus the control group; #p < 0.05 and ##p < 0.01 versus the CPZ group.
Fig 2: Western blot of αSMA, CD31, caveolin-1 and NG2 protein levels in mouse testes treated with 0, 0.5, 1.5 and 2.5 mg/kg CdCl2 daily for 10 days, respectively. (A) Western blot analysis. (B) The semiquantitative density of each protein band. Five mice were used in each group. * p < 0.05; ** p < 0.01; *** p < 0.001.
Fig 3: NG2 immunostaining in mouse testes treated with 0, 0.5, 1.5 and 2.5 mg/kg CdCl2 daily for 10 days, respectively. (A) NG2 immunostaining. (B) The semiquantitative density of NG2 immunostaining. Three mice were used in each group. Bar, 100 μm. *** p < 0.001.
Fig 4: A low GAS5 expression and a high PDGFR α/β expression were observed in the lungs of patients and mice with IPF. A The expressions of GAS5 and PDGFR α/β in the IPF specimens were examined via RT-qPCR. B Western blotting was employed to explore the expressions of desmin, NG2, α-SMA, collagen I, and PDGFR α/β in IPF patients. C The pathological changes in the lung tissues of bleomycin-induced mice were evaluated via H&E staining. D Masson staining was used to determine collagen formation in the lung tissues. E Immunohistochemical staining was employed to detect the expressions of α-SMA and collagen I. F RT-qPCR analysis of the expressions of GAS5 and PDGFR α/β in IPF mice. G Western blotting was used to examine the expressions of desmin, NG2, α-SMA, collagen I, and PDGFR α/β in the lungs. For A and B, n = 33; for C–F, n = 6. *P < 0.05, **P < 0.01, ***P < 0.001
Fig 5: Removal of EPO receptors from OPCs causes a reduction in the number of mature oligodendrocytes and myelination. (A) Representative image of the SVZ zone in tissue from Sox10-tdTomato reporter line mouse at P7, showing the high expression of Sox10 (red) cells in colocalization with Olig2 in this area. Scale bar 200 μm. (B) Representative confocal images of NG2, CC1, and Olig2 immunofluorescence triple staining at P7 in the STR of Sox10-cre;EpoRfx/fx animals. Scale bar: 20 μm. (C) Analysis of the total Olig2+ cell number in Sox10-cre;EpoRfx/fx mice at P7. Unpaired t-test: **P = 0.0058. N = 5 animals per genotype. (D) Analysis of NG2+ cell numbers in Cre+ mice at P7. Unpaired t-test: *P = 0.05, N = 5 animals per genotype. (E) Analysis of the total CC1+ cell number in Sox10-cre;EpoRfx/fx mice at P7. Unpaired t-test: ***P = 0.0002. N = 5 animals per genotype. (F) NG2+/CC1 cells ratio in Sox10-cre;EpoRfx/fx mice shows a 44% vs 29% OL maturation between Cre− and Cre+ mice. Unpaired t-test: ***P < 0.0001. (G) Representative confocal images of MBP and Olig2 immunofluorescence double staining at P7 in the STR of Sox10-cre;EpoRfx/fx animals. Scale bar: 40 μm. (H) Analysis of the total Olig2+ cell number in Sox10-cre;EpoRfx/fx mice at P11. Unpaired t-test: *P = 0.013. N = 5 animals per genotype. (I) Densitometric analysis of MBP expression in the striatum of Sox10-cre;EpoRfx/fx mice at postnatal age P11. Unpaired t-test: **P = 0.0017. N = 5 animals per genotype. (J) Representative confocal images of caspase 3, and Olig2 double immunofluorescence staining in the SVZ at P4 of Sox10-cre;EpoRfx/fx animals. Scale bar: 20 μm. (K) Analysis of Caspase3+/Olig2+ cells in Sox10-cre;EpoRfx/fx mice at P4. Data are given as mean ± SD; Unpaired t-test: **P = 0.0044. N = 4 animals per age and genotype. (L) Western blot representative images of total Erk1/2 and phosphorylated Erk1/2 protein and vinculin as the loading control at P4 in Sox10-cre;EpoRfx/fx mice. (M) Quantification of total Erk1/2 protein. Unpaired t-test. P = 0.24. N = 4 animals per genotype. (N) Quantification of phosphorylated (p-) Erk1/2 protein expression in cre+ and cre− mice at P4. Unpaired t-test, ****P < 0.0001. N = 4 animals per genotype.
Supplier Page from Abcam for Anti-NG2 antibody [EPR23976-145]