Fig 1: CDK7 expression was associated with M2 macrophage infiltration in LUAD. (a) Representative Transwell images and quantification of the relative migration of macrophages after coculture with tumor cells with different levels of CDK7 expression. (b) The IHC showed a positive correlation between CDK7 expression and M2 macrophage infiltration in LUAD tissues.
Fig 2: Detection of antitumor effects in LLC cells in vitro. (a) Confocal images of LLC cells treated with lipo/siCDK7, AuNP@mPEG‐PEI/siCDK7 or AuNP@mPEG‐PEI‐iRGD/siCDK7. SiCDK7 was labeled with Cy5. (b) FCM analysis result of LLC cells after treatment with free siCDK7, lipo/siCDK7, AuNP@mPEG‐PEI/siCDK7 or AuNP@mPEG‐PEI‐iRGD/siCDK7 for 48 h. SiCDK7 was labeled with Cy5. Relative mRNA expression (c) and protein expression (d) of CDK7 in LLC cells after treatment with lipo/siNC, lipo/siCDK7, AuNP@mPEG‐PEI/siCDK7 or AuNP@mPEG‐PEI‐iRGD/siCDK7. (e) CCK‐8 assay results showing the relative viability of LLC cells after exposure to different treatments for 24 h with or without laser irradiation. (f) FCM results showing the apoptosis of LLC cells after exposure to different treatments for 24 h with laser irradiation. (g) CCK‐8 assay results showing the relative viability of LLC cells after exposure to different treatments for 24 h with laser irradiation and necroptosis inhibitor treatment. (h) WB results of the expression of necroptosis markers in LLC cells after exposure to different treatments for 24 h with laser irradiation
Fig 3: CDK7 promoted the immunosuppressive phenotype of macrophages. (a) GSEA plot of the inhibition of FC gamma R‐mediated phagocytosis in the high CDK7 expression group. (b) The expression of factors associated with M1 and M2 polarization in H1975 cells was measured by qPCR. (c) The top 10 enriched KEGG pathways were considerably associated with different genes in THP‐1 cells cocultured with siNC or siCDK7 H1975 cells. (d) THP‐1 cells were cocultured with H1975 cells. The expression of phosphorylated and total AKT and STAT3 in THP‐1 cells was analyzed by western blotting. (e) Heatmap of differentially expressed genes related to cytokine–cytokine receptor interactions in THP‐1 cells cocultured with siNC or siCDK7 H1975 cells. (f) QPCR showed a trend toward an increase in CCL2 and IL‐12b expression in THP‐1 cells cocultured with CDK7‐knockdown H1975 cells. (g) FCM analysis of CD206 expression in RAW264.7 cells cocultured with or without LLC cells. (h) FCM analysis shows the percentage of phagocytic macrophages in a coculture of RAW264.7 cells with LLC cells. RAW264.7 cells were cocultured with LLC cells and were pretreated with an Akt activator (SC79). (i) The expression of phosphorylated and total AKT was analyzed by western blotting in RAW264.7 cells. (j) FCM analysis of CD206 expression in RAW264.7 cells cocultured with LLC cells. (k) FCM analysis shows the percentage of phagocytic macrophages in a coculture of RAW264.7 cells with LLC cells.
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