Fig 1: ASPM-knockdown inhibits the migration, invasion and epithelial-to-mesenchymal transition of hepatocellular cancer cells via KIF11. (A) Migration was detected using a wound healing assay; magnification, x100. (B) Invasion was detected using a Transwell assay; magnification, x100. (C) Protein expression levels of MMP2 and MMP9 were detected by western blotting. Expression levels of N-cadherin, vimentin and E-cadherin were detected by (D) reverse transcription-quantitative PCR and (E) western blotting. **P<0.01; ***P<0.001; n=3. ASPM, assembly factor for spindle microtubules; KIF11, kinesin family member 11; si, small interfering RNA; Ov, overexpression; NC, negative control.
Fig 2: ASPM participates in the Wnt/ß-catenin signaling pathway by regulating KIF11. Expression levels of proteins associated with the Wnt/ß-catenin signaling pathway were detected by western blotting. **P<0.01; ***P<0.001; n=3. ASPM, assembly factor for spindle microtubules; KIF11, kinesin family member 11; si, small interfering RNA; Ov, overexpression; NC, negative control; p, phosphorylated.
Fig 3: DFS analysis, based on Gene Expression Profiling Interactive Analysis, for the hub genes. (A-G) DFS analysis of seven hub genes (BUB1, KIF2C, RACGAP1, CENPE, KIF11, TTK and KIF20A). KIF11, kinesin family member 11; DFS, disease-free survival; HR, hazard ratio; TPM, transcripts per kilobase million.
Fig 4: Kaplan-Meier survival plots. Effect of (A) T stage, (B) Gleason score, (C) KIF11 expression and (D) VEGF expression on survival. VEGF, vascular endothelial growth factor; KIF11, kinesin family member 11.
Fig 5: ASPM-knockdown inhibits the proliferation of hepatocellular carcinoma cells via KIF11. Expression levels of KIF11 were detected by (A) RT-qPCR and (B) western blotting. (C) Proliferation in the si-ASPM and Ov-KIF11 groups was detected using a Cell Counting Kit-8 assay. Expression levels of PCNA and Ki67 were detected by (D) RT-qPCR and (E) western blotting. (F) Colony formation ability was detected using a colony formation assay. *P<0.05; **P<0.01; ***P<0.001 as indicated or vs. si-NC; #P<0.05 vs. si-ASPM + Ov-NC; n≥3. ASPM, assembly factor for spindle microtubules; KIF11, kinesin family member 11; RT-qPCR, reverse transcription-quantitative PCR; si, small interfering RNA; Ov, overexpression; NC, negative control; PCNA, proliferating cell nuclear antigen.
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