Fig 1: ROP1 co-immunoprecipitates with host C1QBP.A. Immunofluorescence verification of C-terminal HA-tagging of ROP1. Scale bar = 10 µm. B. Enrichment of proteins that co-immunoprecipitate with ROP1. Primary MEFs were infected with PRU?KU80 or PRU ROP1-HA parasites for 24 h, following which ROP1 was immunoprecipitated with anti-HA agarose matrix and co-immunoprecipitated proteins were identified and quantified by mass spectrometry. L2FCs were calculated from the geometric mean of the iBAQ intensities across replicates, and p-values calculated by two-sided Welch’s t-test. Proteins with p-value < 0.05 and L2FC > 3 are annotated. C, D. Co-immunoprecipitation of C1QBP with ROP1 in C primary MEFs and D HFFs infected with RH ROP1-HA, PRU ROP1-HA, and PRU 202200-HA. S = post-immunoprecipitation supernatant, IP = immunoprecipitate. Note that the immunoprecipitate fraction represents 3x the relative amount of the total lysate compared to the post-IP supernatant fraction. E, F. Immunofluorescence localisation of ROP1 and C1QBP in RH?KU80 and PRU?KU80 infected E primary MEFs and F HFFs at 24 hours post-infection. Scale bar = 10 µm.