Fig 1: Verification of the expression of genes in the TLR-based signature in HCC and normal tissue specimens. (A) Western blot for detecting MAP2K2, IRAK1, RAC1, TRAF, and MAP3K7 proteins in three paired HCC and normal tissues. (B–G) Quantification of the expression of (B) MAP2K2, (C) IRAK1, (D) RAC1, (E) TRAF, (F) MAP3K7, and (G) SPP1 proteins in three paired HCC and normal tissues. Comparisons between groups were evaluated with Student’s t tests. *p < 0.05; **p < 0.01; ***p < 0.001.
Fig 2: Silencing MAP2K2 weakened colony formation capacity and induced apoptosis of HCC cells. (A, B) RT-qPCR of the mRNA expression of MAP2K2 in Huh7 and HeG2 cells with si-MAP2K2 transfection. (C–E) Colony formation assays of the number of colonies of Huh7 and HeG2 cells with si-MAP2K2 transfection. (F–H) Flow cytometry assays of the apoptotic levels of Huh7 and HeG2 cells under transfection with si-MAP2K2. p values were estimated with ANOVA tests. ***p < 0.001; ****p < 0.0001.
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