Fig 1: Determination of mitochondrial protein levels by Western blot assay. (a) Representative Western blot that hybridized with mitochondrial respiratory complex subunits of SW620 cells. TOM20 was adopted as loading control. MT-ND1, MT-ND4, MT-ND5 and MT-ND6 belong to subunit of complex I; MT-CO2 belongs to subunit of complex IV; MT-CYTB belongs to subunit of complex III. (b) Quantification of mitochondrial protein levels. The optical density was determined using NIH ImageJ software. The values are expressed as percentages of the average values for the control cells. Data are expressed as means ± SD. *, P < .05; **, P < .01, n = 3.