Fig 1: ACAA2 overexpression is associated with a poor prognosis of patients with ovarian cancer. (A and B) Kaplan-Meier progression-free survival curves for patients with a low or high expression of (A) ACAA2 or (B) PNLIP among the total number of patients; (C-E) Kaplan-Meier progression-free survival curves for patients with (C) a negative PAX2 expression (PAX2-) or positive PAX2 expression (PAX2+), (D) low or high ACAA2 expression or (E) low or high PNLIP in expression RAS positive (RAS+) subgroup.
Fig 2: PAX2 promotes fatty acid metabolism reprogramming in RM tumors and ovarian cancer tissues. (A) Western blot analysis of the expression of PAX2, FABP4 and LIPA in RM-WPI or RM-PAX2 tumor tissue, contralateral ovarian and fallopian tube tissues, and from a case of human serous ovarian cancer. SKOV3 and T24 cell lines were used as negative controls for PAX2 expression. (B) Western blot analysis of the expression of PAX2, FABP4 and LIPA in cancer tissues from PAX2 strong (PAX2+++) or weak (PAX2+) serous ovarian cancer patients. Non-cancerous contralateral ovarian and fallopian tube tissues were also analyzed. GAPDH was used as a loading control. Images are representative of 3 replicate experiments. PAX2 and FABP4 were probed from the same membrane, LIPA was probed from another membrane. (C) Western blot analysis of the expression of PAX2, PNLIP and ACAA2 in cancer tissues from an additional six serous ovarian cancer patients. PAX2 and ACAA2 were probed from the same membrane, PNLIP was probed from membrane. PAX2, paired box 2.
Fig 3: iTRAQ proteomics profiling of RM tumors overexpressing PAX2. (A) An overview of the workflow employed in this study. Two iTRAQ replicates were carried out to ensure the consistency and reliability of the results. (B) Expression of PAX2 in allograft RM tumors was confirmed by immunohisto-chemistry. (C) Reproducibility analysis of 2 repeated trials by Pearson's correlation coefficient. (D) Summary of identified and quantified proteins. (E) KEGG pathway-based enrichment analysis of up-regulated proteins (RM-PAX2 vs. RM-WPI). (F) Fatty acid metabolism. Enzymes upregulated by PAX2 overex-pression are indicated in red color. PAX2, paired box 2; Acsl, long-chain-fatty-acid-CoA ligase 1; Cpt1, carnitine O-palmitoyltransferase 1; Cpt2, Carnitine O-palmitoyltransferase 2; Acadl, long-chain specific acyl-CoA dehydrogenase, mitochondrial; Acadvl, very long-chain specific acyl-CoA dehydrogenase, mitochondrial; Acads, short-chain specific acyl-CoA dehydrogenase, mitochondria; Acadm, medium-chain specific acyl-CoA dehydrogenase, mitochondrial; Echs1, Enoyl-CoA hydratase; Ehhadh, peroxisomal bifunctional enzyme; Acaa1, 3-ketoacyl-CoA thiolase A; Acaa2, 3-ketoacyl-CoA thiolase, mitochondrial.
Supplier Page from Abcam for Anti-ACAA2 antibody