Fig 1: TELO2-interacting protein 1 (TTI1) regulates the mTOR signaling pathway and promotes non-small-cell lung cancer (NSCLC) progression in vivo. (A) Protein–protein interaction networks about TTI1 from the STRING database. (B) The Kyoto Encyclopedia of Genes and Genomes analysis explores TTI1-related downstream pathways from the STRING database. (C, D) Quantitative real-time polymerase chain reaction detects TTI1, mTOR, and downstream signaling genes' mRNA levels, including S6K1 and 4EBP1. (E) Western blot analysis of TTI1, mTOR, p-mTOR, S6K1, p-S6K1, 4EBP1, and p-4EBP1 protein levels. (F) Image of harvested subcutaneous tumors that A549-shTTI1-1, H460-TTI1-1, and control cells were implanted into the nude mice. (G) The tumor volume was calculated every 4 days after it reached 100 mm3. (H) The prognosis of those nude mice subcutaneously implanted with A549-shTTI1, H460-TTI1, and negative control cells. The data are presented as mean ± SD, n = 3; *P < 0.05, **P < 0.01, ***P < 0.01; ns, not significant