Fig 1: In vitro binding of RXRa, TP53 and YY1 to biotinylated single strand DNA molecule mimic of TG2-lncRNA, as detected by Western blotting of the associated protein complexes. Experimental details are reported in Material and Methods. The top picture (INPUT) refers to nuclear extracts (10 µL of input) from the mentioned cell lines used in each reaction of in vitro binding assay. NB4 cells represent positive control of RXRa. MCF-7 NC and MDA-MB-231 NC are control reactions in which the extracts were incubated with beads without biotinylated mimic molecule. Hybridization was carried out with specific antibody against RXRa, TP53 and YY1. In the bottom panel, we have compared (OUTPUT) reactions performed using the nuclear extracts after binding with the biotinylated probe. In addition, extracts from NBA cells untreated or induced with 1 and 3 µM ATRA as positive control.
Fig 2: Circ-RCCD inhibits YY1 nucleus translocation. (A) RT-qPCR and (B) Western blot assays evaluated the expression of YY1 in P19 cells with NC OE or circ-RCCD OE or NC KD or circ-RCCD KD transfection. (C) FISH assay displayed that circ-RCCD was mainly located in the cytoplasm. Bar = 100 µm. (D) Western blot assays evaluated the expression of YY1 in cytoplasm or nuclear
Fig 3: Circ-RCCD interacts with transcription factor YY1 to reduce MyD88 expression. (A) Dual-luciferase assay assessed the luciferase activity between YY1 and MyD88. **p < 0.01 vs. NC OE group. (B) CHIP and (C) RIP assays further confirmed the binding between YY1 and MyD88. ***p < 0.001 vs. NC OE group. (D) catRAPID showed that circ-RCCD could bind to MyD88. (E) RIP and (F) RNA pull down assays identified the binding sites between circ-RCCD and MyD88. ***p < 0.001 vs. Input group
Supplier Page from Abcam for Anti-YY1 antibody