Fig 1: The expression levels of peroxiredoxin (PRDX) 1 and 2 in canine hemangioma (HA) and hemangiosarcoma (HSA) tissues. (A) Immunohistochemical (IHC) staining image for PRDX1 expression in canine HA. Macrophages in HA tissues were positive for PRDX1 (arrow). HA cells were negative for PRDX1. IHC score of this sample is 0.00. Bar, 50 µm. (B) IHC staining image for PRDX2 in canine HA. Some erythrocytes were positive for PRDX2 (arrows). HA cells were negative for PRDX2. IHC score of this sample is 0.00. Bar, 50 µm. (C) IHC staining image for PRDX1 in canine HSA. Macrophages (arrow) and neutrophils (arrowhead) were observed as positive and negative control for PRDX1, respectively. HSA cells were positive for PRDX1. IHC score of this sample is 12.00. Bar, 50 µm. (D) IHC staining image for PRDX2 in canine HSA. Some erythrocytes stained positively (arrow) and neutrophils (arrowhead) were used as positive and negative control for PRDX2, respectively. HSA cells were positive for PRDX2. IHC score of this sample is 12.00. Bar, 50 µm. (E) The box-and-whisker plot of IHC scores of PRDX1 in canine HA and HSA. IHC score of PRDX1 in HSA was significantly higher than that in HA. Bar crossing the box-and-whisker plot expresses the sample average. (F) The box-and-whisker plot of IHC scores of PRDX2 in canine HA and HSA. IHC score of PRDX2 in HSA was significantly higher than that in HA. Bar crossing the box-and-whisker plot expresses the sample average. *P<0.05.
Fig 2: Staining properties of peroxiredoxin (PRDX) 1 in normal canine tissues. (A) Squamous cells in the epidermis. The nuclei of squamous cells located in the basal and spinous layers were strongly positive for PRDX1 and the cytoplasm was weakly positive. However, squamous cells in the granular layer were negative for PRDX1. Bar, 20 µm. (B) Squamous cells of hair follicles (arrows) and sebaceous glands (arrowhead). The nuclei of squamous cells were positive for PRDX1 and the cytoplasm was weakly positive. Almost all sebaceous gland cells were positive for PRDX1 expression. Bar, 50 µm. (C) Apocrine sweat glands. Glandular epithelial cells were positive for PRDX1, but myoepithelial cells surrounding the glands were negative. Bar, 20 µm. (D) Macrophages in the dermis. The nucleus and cytoplasm of macrophages were labeled with PRDX1 (arrow). Bar, 20 µm. (E) Vascular vessels in the dermis. Almost all the endothelial cells in the vascular vessels were negative for PRDX1. Bar, 20 µm. (F) Fibroblasts in the dermis. Fibroblasts were negative to positive for PRDX1. Bar, 20 µm. (G) Striated cutaneous muscle. The nucleus and cytoplasm of striated muscle were negative for PRDX1 expression. Bar, 50 µm. (H) Neutrophil in the spleen. Both of the nucleus and cytoplasm of neutrophils were negative for PRDX1 (arrow). Bar, 20 µm. (I) Lymphocytes in the lymphoid follicles of the spleen. The cytoplasm of the lymphocytes was positive for PRDX1. Bar, 20 µm. (J) Erythroblasts in the spleen. Erythroblasts were positive for PRDX1 expression. Bar, 20 µm. (K) Endothelial cells of splenic sinusoids. The nuclei of the endothelial cells of the splenic sinusoid were negative to weakly positive for PRDX1. Bar, 20 µm.
Fig 3: The expression of peroxiredoxin (PRDX) 1 and 2 of canine hemangiosarcoma (HSA) between spleen (n=27) and skin (n=27). (A) The box-and-whisker plot of immunohistochemical (IHC) scores of PRDX1 of canine HSA in spleen and skin. There were no significant differences in the expressions of PRDX1 between spleen and skin. (B) The box-and-whisker plot of IHC scores of PRDX2 of canine HSA in spleen and skin. There were no significant differences in the expressions of PRDX2 between spleen and skin. Bar crossing the box-and-whisker plot expresses the sample average.
Fig 4: The expression of peroxiredoxin (PRDX) 1 and 2 in histopathological subtypes of canine hemangiosarcoma (HSA). (A) The box-and-whisker plot of the immunohistochemical (IHC) scores of PRDX1 in each histopathological subtypes of canine HSA. There were no significant differences in the expressions of PRDX1 among three subtypes of HSA. (B) The box-and-whisker plot of IHC scores of PRDX2 in each histopathological subtypes of canine HSA. There were no significant differences in the expressions of PRDX2 among three subtypes. Bar crossing the box-and-whisker plot expresses the sample average.
Fig 5: The staining properties of peroxiredoxin (PRDX) 1 and 2 in the newly formed vascular vessels of canine granulation tissues. (A) The nucleus and cytoplasm of endothelial cells in angiogenic region were positive for PRDX1 (arrows). Bar, 50 µm. (B) The nucleus and cytoplasm of endothelial cells in angiogenic region were positive for PRDX2 (arrows). Bar, 50 µm. (C) The nucleus and cytoplasm of endothelial cells in resting region were negative for PRDX1 (arrows). Bar, 50 µm. (D) The nucleus and cytoplasm of endothelial cells in resting region were negative for PRDX2 (arrows). Bar, 50 µm.
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