Fig 1: Brain developmental disorders with ventriculomegaly and cortical atrophy.(A) Viral loads in different organs. Viral genome copy number was assessed by qPCR using MCMV-DNA extracted from equal amounts of different organ tissues from 3 MCMV-infected mice at the indicated time points. Results are presented as mean ± SEM. (B) Dynamic changes in brain weight. Representative images of brains harvested at P7 using bright-field and fluorescence microscopies are shown. Hemorrhage is indicated by black arrow. Brain weights were measured at the indicated time points. Scale bar: 2 mm. (C) Lateral ventricle areas and thicknesses of cortical layers at P7. Coronal brain sections were stained for IE1 (green), DAPI (blue), and Tbr1 (red) or Ctip2 (red), respectively. Lateral ventricle areas of position-matched brain sections outlined by white dashed lines were measured. Thicknesses of cortical layers in the position-matched region (indicated in white dashed box) were measured. Data were collected from 3 mice/group in 3 independent experiments. Results are presented as mean ± SEM and analyzed by 2-tailed Student’s t test, respectively. *, P < 0.05; **, P < 0.01, ***, P < 0.001. Scale bar: 1 mm or 100 µm in the magnified images.