Fig 1: Different staining analysis of the same vascular area in pheochromocytoma tissue. (A, B) Hematoxylin and eosin staining showed the vascular lumen area. (C–H) Immunostaining of COX4I2, α-SMA and CgA was performed in the same area. (I–X). Immunofluorescence staining of COX4I2, α-SMA, DAPI and CgA was performed in the same area.
Fig 2: Single cell atlas of pheochromocytoma. (A) Two-dimensional t-SNE clustering analysis showed that the cells were divided into 21 clusters and labeled with different colors. (B, C) The expression of TH and CHGA genes was used to identify pheochromocytoma tumor cells. (D) The expression of PTPRC gene was used to identify immune cells. (E–G) The expression of VWF, PECAM1 and PLVAP genes was used to identify endothelial cells. (H, I) The expression of ACTA2 and PDGFRB genes was used to identify fibroblasts. (J) Location and expression of COX4I2 gene in all cells.
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