Fig 1: Western blotting and quantified analysis of renal injury biomarkers. (A) Representative blots of NGAL and KIM-1 protein expression. (B) Quantification on NGAL relative expression. (C) Quantification on KIM-1 relative expression. (D) The expression of NGAL was detected by immunohistochemistry. Row magnification, x100 (scale bar, 50 µm). (E) Quantification on the positive area. *P<0.05, **P<0.01, ***P<0.001 vs. SHAM group; #P<0.05, ###P<0.001 vs. CM-IRI group). NGAL, neutrophil gelatinase-associated lipocalin; CM, contrast media; IRI, ischemia reperfusion injury.
Fig 2: The effect of Glab on renal function. A H&E, B PAS, and C Sirius red staining of renal tissues (magnification 200 × ; bar = 100 µm). D Alloknesis score was recorded to evaluate mechanical itch behavior. E Kidney weight and (F) index. The renal function was assessed by detecting (G) serum creatinine (Scr), (H) blood urea nitrogen (BUN), and (I) urinary albumin excretion rate (UAER). J Kidney damage biomarkers (KIM-1, NGAL, and TIMP-1) in urinary excretion were detected by western blot (***P < 0.005, vs. the control group; ##P < 0.01 and ###P < 0.005, vs. the DM group)
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