Fig 1: Upregulated miR-133a inhibited the proliferation, invasion and migration of glioblastoma cells, which could be reversed by upregulated TGFBR1. TGFBR1 expression in U87 and U251 cells was detected using qRT-PCR (A) and WB (B); C The proliferation ability of U87 and U251 cells was detected by CCK-8 detection; D The invasion ability of U87 and U251 cells was detected by Transwell assay; E The migration ability of U87 and U251 cells was detected by wound healing test. *represented comparison with mimic NC, p < 0.05; + represented comparison with miR-133a mimic + pcDNA3.1, p < 0.05. n = 3. Data were expressed as mean ± standard deviation. One-way ANOVA was used for comparisons between multiple groups, comparison between two groups are followed by t test with Bonferroni correction
Fig 2: miR-130a’s impact on TGF-ß/Smad signaling in CFs under hypoxia. (a) Col-1, p-Smad3, t-Smad3, a-SMA, TGF-ß, and TGFBR1 protein levels in CFs under hypoxia, as determined with Western blot (n = 6 per group). (b-d) Col-1, TGF-ß, and TGFBR1 mRNA levels in CFs under hypoxia, as determined with rt-PCR (n = 6 per group).
Fig 3: Attenuation of the influence of miR-130a by co-transfection with TGFBR1. (a) Col-1 and a-SMA protein levels, as determined with Western blot. (b-c) Col-1 and a-SMA mRNA levels, as determined with rt-PCR.
Fig 4: Tim-1 regulated the expression of TGFBR1. Genes with significantly differential expression in U87 and U251 (A) cells after Tim-1 downregulation; the scatter diagram of GO function enrichment analysis results in U87 and U251 (B) cells after Tim-1 downregulation; the scatter diagram of KEGG enrichment analysis results in U87 (A) and U251 (B) cells after Tim-1 downregulation; Genes with significantly differential expression both in U87 and U251 cells (C)
Fig 5: miR-130a’s impact on TGF-ß/Smad signaling in CFs under normoxia. (a) Col-1, TGFBR1, p-Smad3, t-Smad3, a-SMA, and TGF-ß protein levels in CFs under normoxia, as determined with Western blot (n = 6 per group). (b-d) Col-1, TGF-ß, and TGFBR1 mRNA levels in CFs under normoxia, as determined with rt-PCR (n = 6 per group).
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