Fig 1: CLDN7 overexpression suppresses the proliferation of oral squamous cell carcinoma cells. (A) CLDN7 expression in CAL-27 cells was determined via western blotting after transfection with a CLDN7 overexpression plasmid. (B) Cell viability was detected using a Cell Counting Kit-8 assay. (C and D) A colony formation assay was performed to determine the proliferative abilities of CAL-27 cells. **P<0.01, ***P<0.001 vs. pcDNA3.1. CLDN7, Claudin-7.
Fig 2: CLDN7 knockdown reverses the inhibitory effects of IRF2 overexpression on cell proliferation in OSCC cells. (A) Knockdown efficiency of CLDN7 was demonstrated using western blotting. (B) A Cell Counting Kit-8 assay was used to evaluate cell viability in the Control group, pcDNA3.1 group, pc-IRF2 group, pc-IRF2 + shNC group and pc-IRF2 + shCLDN7 group. (C and D) Colony formation assays were used to assess the number of cell colonies in the Control group, pcDNA3.1 group, pc-IRF2 group, pc-IRF2 + shNC group and pc-IRF2 + shCLDN7 group. *P<0.05, ***P<0.001 vs. pcDNA3.1; ##P<0.01, ###P<0.001 vs. pc-IRF2 + shNC. CLDN7, Claudin-7; IRF2, interferon regulatory factor-2; OSCC, oral squamous cell carcinoma; sh, short hairpin RNA; NC, negative control.
Fig 3: CLDN7 overexpression inhibits the invasion and migration of oral squamous cell carcinoma cells. (A and B) The invasive ability of CAL-27 cells was measured using a Transwell assay following CLDN7 overexpression. (C and D) The cell migratory ability of CAL-27 cells was detected using a wound healing assay. ***P<0.001 vs. pcDNA3.1. CLDN7, Claudin-7.
Fig 4: IRF2 overexpression upregulates the expression of CLDN7 in OSCC cells. (A) IRF2 expression in several OSCC cells (SCC-9, CAL-27, SCC-15 and SCC090) and the human oral keratinocyte cell line HOK was detected via (A) RT-qPCR and (B) western blotting. ***P<0.001 vs. HOK. (C) IRF2 expression was evaluated using RT-qPCR following transfection with IRF2 plasmid in CAL-27 cells. ***P<0.001 vs. pcDNA3.1. (D) Western blotting was used to assess the expression of CLDN7 following IRF2 overexpression. ***P<0.001 vs. pcDNA3.1. CLDN7, Claudin-7; IRF2, interferon regulatory factor-2; RT-qPCR, reverse transcription-quantitative PCR; OSCC, oral squamous cell carcinoma.
Fig 5: CLDN7 knockdown reverses the inhibitory effects of IRF2 overexpression on cell migration and invasion in OSCC cells. (A and B) Invasive ability of CAL-27 cells in the Control group, pcDNA3.1 group, pc-IRF2 group, pc-IRF2 + shNC group and pc-IRF2 + shCLDN7 group was measured using a Transwell assay. (C and D) Migration rates of CAL-27 cells in the Control group, pcDNA3.1 group, pc-IRF2 group, pc-IRF2 + shNC group and pc-IRF2 + shCLDN7 group was detected using a wound healing assay. ***P<0.001 vs. pcDNA3.1; ###P<0.001 vs. pc-IRF2 + shNC. CLDN7, Claudin-7; IRF2, interferon regulatory factor-2; OSCC, oral squamous cell carcinoma; sh, short hairpin RNA; NC, negative control.
Supplier Page from Abcam for Anti-Claudin 7/CLDN-7 antibody