Fig 1: The probiotic cocktail repaired the gut barrier and reduced inflammatory factors in DSS-induced colitis. (A–E) The level of serum inflammatory cytokines (pro-inflammatory IFN-gamma, IL-17, and IL-23; anti-inflammatory IL-10 and IL-4) in blank mice, and DSS-induced colitis mice treated with PBS, healthy mice donor FMT, 5-ASA, or the probiotic cocktail. (F–H) The mRNA expression of tight junction protein (JAM-1, Occludin, ZO-1) of colon tissues in each group. (I) The protein expression of tight junction protein (JAM-1, Occludin, ZO-1) of colon tissue in each group. One-way ANOVA test was utilized, and data were shown as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, no significant difference. DSS, dextran sodium sulfate; PBS, phosphate buffered saline; FMT, fecal microbiota transplantation; 5-ASA, 5-aminosalicylic acid.