Fig 1: Organ failure induced by LA in mice(A–F) Box plots of control (Con) or LA treated mice comparing carotid artery pulse distention (Pulse Dist (PD); A) as a measure of shock, Rectal temperature (RT; B) as a measure of severe inflammatory response syndrome, blood urea nitrogen (BUN; C), creatinine (Creat.; D) as a measure of renal failure. TUNEL positive cells/ high power field in the renal cortex (E), and lung (F) as measures of cell death in these organs.(G–J) The images are representative TUNEL stained histologic sections, showing brown TUNEL positive cells. In the kidney panels (G and H), the yellow arrows show some of the TUNEL positive nuclei in renal tubules, and the dashed outlines show some examples of the dilated lumina of tubules, with loss of brush border. In the lung panels (I and J), black arrows denote the TUNEL positive cells in the blood vessels and red arrows denote the positive cells in the alveoli.(K–N) (K) Ratio of lactate dehydrogenase (LDH) activity by protein content in the bronchoalveolar lavage (BAL) fluid collected at the time of necropsy. L-N show annexin V positive (AxV+) staining in all BAL cells (L), Thyroid transcription factor-1 (TTF-1) positive (M), or CD208 positive cells (N).(O) shows representative flow cytometry scattergrams of BAL cells stained for CD208 and annexin V. p values calculated by a Mann-Whitney U test are shown above corresponding box plots.