Fig 2: MiR-23a-3p suppressed sorafenib-induced ferroptosis. A Dot plot showing KEGG pathway enrichment for differentially expressed proteins among NC + sorafenib, NC, and miR-23a-3p groups. B Heatmap showing protein expression pattern with KEGG pathway annotation. C The protein expression of GPX4 and ACSL4. D Chelatable iron accumulation was detected by fluorescent indicator Phen Green SK with dynamic quenching signals. E The deposition of lipid peroxides was stained with BODIPY and measured by LSM780 confocal imaging. F Cell viability of HCC cells examined by MTT assay

Fig 3: Puerarin suppresses LPS-induced myocardial ferroptosis via up-regulation the phosphorylation of AMPK. (A, B) Representative Western blot analysis and quantitative protein analysis of GPX4 and ACSL4 expression in cardiac protein extract. (C, D) Representative Western blot analysis and quantitative protein analysis of Ferritin and TFR expression in cardiac protein extract. (E, F) Representative Western blot analysis and quantitative protein analysis of P-AMPK expression in cardiac protein extract. (G) represents the amount of iron in the heart. Data represent the mean ± SD. *P < 0.05 vs Control group; #P < 0.05 vs. LPS group; &P < 0.05 vs. LPS+Pue group.

Fig 4: General view of the effect of HMSeBA on intestinal ER stress. A model for HMSeBA supplementation regulates ER stress induced by LPS or H2O2 in vivo and vitro. Only core components of the pathway are shown. LPS or H2O2 administration induce ER stress through activating the GRP78-IRE1α-CHOP signaling pathway. While HMSeBA treatment improves the expression of GPX4 and SELS, thereby suppresses the ER stress signal, ameliorates the redox status. Maternal HMSeBA is involved in regulating the ER stress signal and health of intestine.

Fig 5: The expression of TH and GPX4 was decreased, while the expression of SNX5 was increased in the PD cell model. (a) PC-12 cells were untreated or treated with 40 μM 6-OHDA for 24 h. The expression of TH, GPX4, and SNX5 was detected by Western blotting. (b) MDA Assay was used to detect the concentration MDA in PC-12 cells was induced by 6-OHDA. (c) GSH/GSSG ratio in vitro PD was detected by GSH/GSSG Assay. Data were expressed as mean ± S.D.; three independent replicates of the experiment (∗∗∗p < 0.05).