Fig 1: MBNL1 regulates circSTAU2 Expression. (A) Overlapping analysis of the predicted RBPs from RBPdb and RBPmap databases. (B) qRT-PCR analysis of circSTAU2 expression in MKN45 and BGC823 cells transfected with siMBNL1 or siKHDRBS3. (C) The predicted binding site between MBNL1 and STAU2 pre mRNA from RBPdb database. (D) qRT-PCR analysis of the enrichment of predicted binding sites with the isolated co-precipitated RNA by MBNL1-RIP, and DNA electrophoresis was performed with the PCR products. (E) The RNA-FISH assay with an antisense probe and immunofluorescence with MBNL1 antibody were conducted to detect the expression and localization of circSTAU2 and MBNL1 in GES-1, MKN45 and BGC823 cells and (F) MBNL1-overexpressed MKN45 cells. Data are expressed as mean ± SD. ***p<0.001.