Fig 1: IDH1R132H promotes malignant transformation of prostate benign epithelial cell RWPE-1. (A, B) Western blot and qRT-PCR analysis of protein and mRNA expression levels of wild-type IDH1 and IDH1R132H in RWPE-1cells, following stably expressing empty vector, wild-type, or mutant IDH1R132H. (C) IDH1R132H enhances the cytokine-independent growth of benign prostatic epithelial cells. The growth of stable RWPE-1 cells expressing IDH1R132H, IDH1WT, and VECTOR was examined by MTS assay. Cells were cultured under cytokine-poor conditions (1/4 normal dose EGF and BPE). (D, E) IDH1R132H promotes migration of benign prostatic epithelial cells. The migration of IDH1R132H on RWPE-1 cells was examined by wound-healing assay and Transwell migration assay. (F, G) The growth and migration of RWPE-1 cells. Cells were treated with 20 μM AGI-5198 or DMSO and analyzed using MTS and Transwell migration assay. All data represent mean ± SD of at least three independent replicates. *P < .05, **P < .01.