Fig 1: MBC subsets in SARS-CoV-2 infection(A and B) Confocal images of lung sections from (A and B) K18-hACE2 or (B) wild-type mice infected with 2.103 PFU of SARS-CoV-2. Scale bars, 130 µm.(C and D) Contour plots showing (C) lung and (D) lymph node class-switched resident B cells (left) and MBCs (middle) from mice infected as in (A) and analyzed at day 70. The multicolor bar (right) shows IgH isotype distribution in MBCs.(E and F) Dot plots showing the presence of MBC subsets in (E) lungs and (F) lymph node.(G and H) Contour plots showing MBC binding to Spike protein in (G) lungs and (H) lymph node.(I–K) Confocal images of lung sections at day 70 of the infection. Scale bars: 25 µm in (I), 18 µm in (J), and 20 µm in (K). The quantification in (I) displays the minimal distance of B cell clusters to EpCAM+ cells; dots represent B cell clusters. The quantification in (J) displays the minimal distance (nm) of IgM+ or IgG2b+ cells to the B cell cluster border divided by the cluster’s area (µm2); dots represent individual B cells.(L) Dot plot showing the area and B220 fluorescence for the detected IgG2b+ surfaces in (K) (left). Dot plot showing CCR6 and CXCR3 fluorescence intensity for IgG2b+ MBCs (center). X and Y positions of MBC subsets are laid out in the B220 representation (right).In (E)–(H), bar charts show the quantification of one experiment out of three, mean ± SEM. Each dot represents one mouse. (E and F) Two-way ANOVA test and (G and H) one-way ANOVA test. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.See also Figure S6.