Fig 1: Expression levels of members in the NF-?B signaling pathway. (A) Expression levels of NF-?B-p65 and I?B-a proteins were determined by Western blot analysis using specific primary antibody and HRP-labeled anti-rabbit IgG antibody. (B) Band intensities were determined using an imaging densitometer, and protein expressions were calculated relative to the intensity of ß-actin. Tissue samples were collected from 3 to 5 mice per group, and each lysate was analyzed in duplicate for Western blot (final n = 6–10). Data are reported as the mean ± SD. * indicates p < 0.05 compared to the WT mice.