Fig 2: TP53INP1 is a direct target of miR-155-5p in OSCC cells. (A) Three different miRNA prediction algorithms (miRDB, Targetscan, and miRWalk) revealed miR-155-5p targets candidates. (B) The mRNA expression levels of miR-155-5p targets candidates in HSC3, HSC3/5-FU, HSC3/5-FU+Anti-miR-155-5p cells. (C) The mRNA and (D) protein levels of TP53INP1 in HSC3 and HSC4 cells infected with vectors expressing miR-155-5p mimic or miR-155-5p mimic +anti-miR-155-5p (rescue). (E) The prediction of the binding between miR-155-5p and TP53INP1 by Pictar. (F) Relative luciferase activity of the indicated TP53INP1 reporter constructs in HSC3 cells. *p < 0.05, **p < 0.01.

Fig 3: MiR-155-5p enhanced 5-FU resistance through targeting TP53INP1 in OSCC. After 5-FU treatment, (A) IC50 of 5-FU, (B) the wound-healing assay, (C) the transwell migration assay, and (D) the transwell invasion assay in HSC3 and HSC4 cells transfected with mock, miR-155-5p mimic, miR-155-5p+TP53INP1 (rescue) or TP53INP1 over-expression plasmid. Scale bar, 100 µm. *p < 0.05, **p < 0.01, *** p < 0.001.