Fig 1: Effects of ceranib-2 and SKI-II on rpS6, eIF4E, MNK1, and IKK in MV-infected PBL. In (A), Western blotting was performed to detect phosphorylated rpS6, total rpS6 protein, and GAPDH. The quantification with normalization to the corresponding DMSO control values for each time point is shown on the right side. The values for phosphorylated over total rpS6 and total rpS6 protein over GAPDH signals are given. The increase of total rpS6 in comparison with the DMSO control was significant at 16 and 24 h after infection. The 16 and 24 h values of ceranib-2-treated infected cells and the values of 6, 16, and 24 h SKI-II-treated infected cells were significantly reduced in comparison with both, uninfected and infected cell values. The phosphorylation of rpS6 was significantly reduced by SKI-II at 16 and 24 h in comparison with infected cell values (n = 3; with *p = 0.05). In (B), Western blotting was performed to detect phosphorylated eIF4E, total eIF4E protein, and GAPDH. The quantification with normalization to the corresponding DMSO control values for each time point is shown on the right side. The values for phosphorylated over total eIF4E signals are given. Significances were calculated by student’s t-test by comparing corresponding time points of inhibitor treated with control signals (n = 3; with *p = 0.05). In (C), Western blotting was performed to detect phosphorylated MNK1, total MNK1 protein, and GAPDH. Only the values for total MNK1 over GAPDH signals are given, because there were no significant differences in the phosphorylation of MNK1. Significances were calculated by student’s t-test by comparing corresponding time points of inhibitor treated with control signals (n = 3; with *p = 0.05). In (D), Western blotting was performed to detect total IKK protein and GAPDH. The quantification with normalization to the corresponding DMSO control values for each time point is shown on the right side. The values for total IKK over GAPDH signals are given. Significances were calculated by student’s t-test by comparing corresponding time points of inhibitor treated with control signals (n = 3; with *p = 0.05). Some blots were used for detection of two different proteins (MNK1 and IKK) and GAPDH controls were re-used, and in some cases, two blots with lysates from the same experiment were used for the detection of total and phosphorylated proteins. The corresponding GAPDH controls were used for quantification. Only blots from one representative experiment are shown.