Fig 1: Expression of regeneration-associated proteins in DRG neurons in CSP-TTK21-treated mice in a chronic SCI.(A-N) Representative micrographs of immunostaining for regeneration-associated proteins (green, white arrows) in DRG neurons from CSP or CSP-TTK21-treated mice (A, C, E, G, I, K, M) and respective quantification of immunostaining in DRG neurons (B, D, F, H, J, L, N). (A, B) Atf3 (CSP: 2,113.0 ± 461.5; CSP-TK21: 4,614.0 ± 449.4, p < 0.05). (C, D) Sprr1a (CSP: 4,904.0 ± 1,033.0; CSP-TK21: 9,331.0 ± 914.6, p < 0.05). (E, F) cJun (CSP: 3,697.0 ± 302.2; CSP-TK21: 4,904.0 ± 340.7, p < 0.05). (G, H) Klf7 (CSP: 4,097.0 ± 199.1; CSP-TK21: 772.00 ± 317.9, p < 0.0001). (I, J) Gap43 (CSP: 1,178.0 ± 173.2; CSP-TK21: 4,643.0 ± 163.6, p < 0.001). (K, L) pErk (CSP: 3,433.0 ± 428.9; CSP-TK21: 3,036.0 ± 750.7, p = 0.66, TOST: t(4.8) = 0.03, p = 0.51 given equivalence bounds of -366.7 and 366.7 on a raw scale and alpha of 0.05). (M, N) pStat3 (CSP: 2,894.0 ± 470.1; CSP-TK21: 3,579.0 ± 656.8, p = 0.43, TOST: t(5.4) = -0.42, p = 0.66 given equivalence bounds of -342.7 and 342.7 on a raw scale and alpha of 0.05). Mean ± SEM; unpaired two-tailed Student or Welch t test; *p < 0.05, *** p < 0.001, **** p < 0.0001. n = 3 or 4 biologically independent animals. The data can be found in S1 Data. CSP, carbon nanosphere; DRG, dorsal root ganglion; SCI, spinal cord injury; TOST, two one-sided tests.