Anti-ICAM1 antibody [15.2] ab20 from Abcam

Product Specs
ItemAnti-ICAM1 antibody [15.2]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab20
Antibody TypePrimary
HostMouse, Nude mouse
ClonalityMonoclonal
Clone15.2
IsotypeIgG1
ApplicationsFlow Cytometry, Immunohistochemistry (Frozen sections), Immunoprecipitation, Western blot
Conjugate/TagUnconjugated
Gene NameIcam1
ReactivityHuman
TargetICAM1
Research AreaImmuno-oncology
Concentration0.8 mg/ml
Quantity100 UG

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Tyrosol attenuates lipopolysaccharide‑induced inflammation in HUVECs to promote vascular health against atherosclerosis challengeExperimental and Therapeutic MedicineApril 6, 2023Weiwei Zhao, Huizhen Wei, Jun Lu, Wenjun Sha, Dusang Sun, Ting Pan, Tao Leitemperature for 2 h. The antibodies used were as follows: Mouse anti-ICAM-1 (dilution, 1:1,000; cat. no. ab20), rabbit anti-VCAM-1 (dilution, 1:1,000; cat. no. ab181315), rabbit GAPDH (dilution, 1:1,000; cat. no.

(2) The Effects of the CXCR4 Antagonist, AMD3465, on Human Retinal Vascular Endothelial Cells (hRVECs) in a High Glucose Model of Diabetic RetinopathyMedical Science MonitorSeptember 16, 2019Di Wu, Li Jin, Hongshuang Xu(ab1670; Abcam, Cambridge, MA, USA); SDF-1 (1 µg/ml) (ab18919; Abcam, Cambridge, MA, USA); ICAM1 (1: 1000) (ab20; Abcam, Cambridge, MA, USA); VCAM1 (1: 1000) (ab98954; Abcam, Cambridge, MA, USA); VEGF (1: 2000) (ab53465;

(3) Peroxisome Proliferator-Activated Receptor-γ Antagonizes LOX-1-Mediated Endothelial Injury by Transcriptional Activation of miR-590-5pPPAR ResearchJuly 1, 2019Lei Xu, Gang Zhao, Hong Zhu, Shijun Wang, Aijun Sun, Yunzeng Zou, Junbo Geantibody at 1:1000 dilution, ab203246, Abcam; anti-ICAM-1, mouse monoclonal antibody at 1:1000 dilution, ab20, Abcam; anti-VCAM-1, rabbit monoclonal antibody at 1:1000 dilution, ab 174279, Abcam; and anti-p47phox

(4) Binding Heterogeneity of Plasmodium falciparum to Engineered 3D Brain Microvessels Is Mediated by EPCR and ICAM-1mBioMay 28, 2019Bernabeu M, Gunnarsson C, Vishnyakova M, Howard CC, Nagao RJ, Avril M, Taylor TE, Seydel KB, Zheng Y, Smith JD.perfusion by gravity-driven flow with mouse anti-human ICAM-1 monoclonal antibody (MAb) 15.2 (Abcam; ab20 [5 μg/ml]), rat anti-human EPCR MAb 252 (Sigma-Aldrich; E6280-200UL [50 μg/ml]), or mouse (Abcam; ab19443)

(5) Protective Effects of Total Saponins of Aralia elata (Miq.) on Endothelial Cell Injury Induced by TNF-α via Modulation of the PI3K/Akt and NF-κB Signalling PathwaysInternational Journal of Molecular SciencesDecember 21, 2018Ping Zhou, Weijie Xie, Yun Luo, Shan Lu, Ziru Dai, Ruiying Wang, Guibo Sun, Xiaobo Sunkits were(ab59348), obtained BAX (ab32503), cleaved-caspase-3 (ab4051), cleaved-caspase-9 (ab52298), cytoC (ab13575), ICAM-1 from Pierce Biotechnology (IL, USA). Antibodies against p-Akt (ab131443), Akt (ab106693), BCL-2 (ab20), Bad (ab32445), Bcl-xl (ab32370), iNOS (ab3523) and eNOS (ab76198) were obtained from (ab59348), BAX

(6) Tissue Damage Caused by Myeloablative, but Not Non-Myeloablative, Conditioning before Allogeneic Stem Cell Transplantation Results in Dermal Macrophage Recruitment without Active T-Cell InteractionFrontiers in ImmunologyFebruary 27, 2018Peter van Balen, Boris van der Zouwen, Alwine B. Kruisselbrink, Matthijs Eefting, Karoly Szuhai, Ekaterina S. Jordanova, J. H. F. Falkenburg, Inge Jedemaagainst HLA class II (IgG1, p104630, Dako, Heverlee, Belgium), HLA-DR (IgG2b, B8.11.2), and CD54 (IgG1, ab20, Abcam) as markers for activated cells. To investigate whether HLA class II-positive cells in skin biopsies

(7) Puerarin protects endothelial progenitor cells from damage of angiotensin II via activation of ERK1/2‑Nrf2 signaling pathwayMolecular Medicine ReportsDecember 19, 2017Chen Fu, Baoxin Chen, Xianglan Jin, Xuemei Liu, Fengli Wang, Rongjuan Guo, Zhigang Chen, Hong Zheng, Le Wang, Yunling Zhang(β‑gal; cat. no. ab168341; 1:500; Abcam), anti‑intracellular adhesion molecule‑1 (ICAM‑1; cat. no. ab20; 1:1,000; Abcam) anti‑vascular cell adhesion molecule‑1 (VCAM‑1; cat. no. ab134047; 1:5,000; Abcam),

(8) Calcium dobesilate may alleviate diabetes-induced endothelial dysfunction and inflammationMolecular Medicine ReportsDecember 1, 2017Yijun Zhou, Jiangzi Yuan, Chaojun Qi, Xinghua Shao, Shan Mou, Zhaohui NiAbcam; cat. no. ab39256), endocan (1:1,000; Abcam; cat. no. ab103590), ICAM‑1 (1:200; Abcam; cat. no. ab20), MCP‑1 (1:5,000; Abcam; cat. no. ab151538) and Figure 2. Western blot analysis of VEGF, VEGFR, endocan,

(9) Hepatic p63 regulates steatosis via IKKβ/ER stressNature CommunicationsAugust 8, 2017Begoña Porteiro et al.difluoride membranes (Millipore, USA) and probed using a primary antibody for Anti-Apolipoprotein B (ab20,737, Abcam, UK) and transferrin I-20 (sc-22,597, Santa Cruz Biotechnology, USA) as loading control. Enzymatic assay

(10) β-Catenin promotes cell proliferation, migration, and invasion but induces apoptosis in renal cell carcinomaOncoTargets and TherapyMarch 7, 2017Chun-Ming Yang, Shan Ji, Yan Li, Li-Ye Fu, Tao Jiang, Fan-dong Mengno. ab104867; 1:1,000), anti-GAPDH (cat. no. 5174; 1:1,500); mouse polyclonal antibody anti-ICAM-1 (cat. no. ab20; 1:500); rabbit polyclonal antibody anti-VCAM-1 (cat. no. ab174279; 1:5,000). All the primary antibodies

(11) Release of HIV-1 sequestered in the vesicles of oral and genital mucosal epithelial cells by epithelial-lymphocyte interactionPLOS PathogensFebruary 27, 2017Aizezi Yasen, Rossana Herrera, Kristina Rosbe, Kathy Lien, Sharof M. Tugizovepithelial cells, we incubated polarized epithelial cells with mouse monoclonal antibodies against ICAM-1 (AB20, clone 15.2, 5 μg/ml, Abcam) or isotype controls for 30 min [126]. At the same time, activated PBMC

(12) Puerarin inhibits M2 polarization and metastasis of tumor-associated macrophages from NSCLC xenograft model via inactivating MEK/ERK 1/2 pathwayInternational Journal of OncologyFebruary 1, 2017Honggang Kang, Jing Zhang, Baozhong Wang, Meirong Liu, Jun Zhao, Mengxiang Yang, Yingxue LiAbcam), anti-VEGF (1:1,000, ab32152; Abcam), anti-MMP-9 (1:100, ab73734; Abcam), anti-ICAM-1 (1:20, ab20; Abcam), anti-MEK (1:20,000, ab178876; Abcam), anti-p-MEK (1:500, ab194754; Abcam), anti-ERK1/2 (1:100, ab54230;

(13) Demethylzeylasteral ameliorates inflammation in a rat model of unilateral ureteral obstruction through inhibiting activation of the NF-κB pathwayMolecular Medicine ReportsJanuary 1, 2017Qiang Wang, Yanmei Xiao, Tongwei Liu, Haibo Yuan, Chunwu LiCell Signaling Technology, Inc., Boston, MA, USA), anti‑intercellular adhesion molecule‑1 (ICAM‑1; 1:3,000; cat. no. ab20; Abcam, Cambridge, UK), anti‑NF‑κ B p65 (1:3,000; cat. no. 8242; Cell Signaling Technology, Inc.) and

(14) Interaction between Endothelial Protein C Receptor and Intercellular Adhesion Molecule 1 to Mediate Binding of Plasmodium falciparum-Infected Erythrocytes to Endothelial CellsMBioJuly 12, 2016Avril M, Bernabeu M, Benjamin M, Brazier AJ, Smith JD.antibodies (1/500; Molecular Probes). For inhibition assays, anti-human ICAM-1 (clone 15.2, 10 µg/ml; Abcam ab20) or isotype control IgG1 (10 µg/ml) antibodies were added 30 min prior to the addition of recombinant

(15) Artemisinin inhibits monocyte adhesion to HUVECs through the NF-κB and MAPK pathways in vitroInternational Journal of Molecular MedicineJune 1, 2016YUE WANG, JIATIAN CAO, YUQI FAN, YUSHUI XIE, ZUOJUN XU, ZHAOFANG YIN, LIN GAO, CHANGQIAN WANGfrom bovine serum for 1 h, the membranes were reacted with primary antibodies, including anti-ICAM-1 (ab20), anti-VCAM-1 (Ab174279) (both from Abcam Cambridge, UK), anti-p65 (#6956), anti-IκB (#4814), anti-ERK

(16) Augmented Dried versus Cryopreserved Amniotic Membrane as an Ocular Surface DressingPLoS ONEOctober 30, 2013Claire L. Allen, Gerry Clare, Elizabeth A. Stewart, Matthew J. Branch, Owen D. McIntosh, Megha Dadhwal, Harminder S. Dua, Andrew Hopkinson

(17) Severe malaria is associated with parasite binding to endothelial protein C receptorNatureJune 27, 2013Turner L, Lavstsen T, Berger SS, Wang CW, Petersen JE, Avril M, Brazier AJ, Freeth J, Jespersen JS, Nielsen MA, et al.pre-incubated for 15 min with either 5 μg/ml of mouse monoclonal anti-human ICAM-1 (clone 15.2, Abcam ab20), 0.1 mg/ml of polyclonal goat anti-EPCR (AF2245, R&D) or 50 μg/ml of activated protein C (Sigma P2200).

(18) Development of a Homogeneous High-Throughput Screening Assay for Biological Inhibitors of Human Rhinovirus InfectionJ Biomol ScreenMarch 1, 2013Philip Newton et al.

(19) DC8 and DC13 var genes associated with severe malaria bind avidly to diverse endothelial cellsPLoS pathogensJanuary 1, 2013Marion Avril, Andrew J Brazier, Martin Melcher, Sowmya Sampath, Joseph D Smithµg/ml, Beckman Coulter Immunotech) or mouse monoclonal anti-human ICAM1 (clone 15.2, 5 µg/ml Abcam ab20). For protein binding inhibition assays, the THBMEC monolayer was pre-incubated for 30 min at 37°C with

(20) A restricted subset of var genes mediates adherence of Plasmodium falciparum-infected erythrocytes to brain endothelial cellsProceedings of the National Academy of Sciences of the United States of AmericaJune 26, 2012Avril M, Tripathi AK, Brazier AJ, Andisi C, Janes JH, Soma VL, Sullivan DJ Jr, Bull PC, Stins MF, Smith JD.

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Anti-ICAM1 antibody [15.2] from Abcam

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Anti-ICAM1 antibody [15.2] from Abcam

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