Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] ab22506 from Abcam

Product Specs
ItemAnti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab22506
Antibody TypePrimary
ClonalityMonoclonal
Quantity10 UG, 100 UG
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections), Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections)
CloneMAC387
IsotypeIgG1
HostMouse, Nude mouse
Conjugate/TagUnconjugated
Concentration1 mg/ml
ReactivityHuman, Mouse
TargetS100A9 + Calprotectin (S100A8/A9 complex)

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(1) Urinary single-cell sequence analysis of the urinary macrophage in different outcomes of membranous nephropathyClinical Kidney JournalNovember 30, 2023Xi Liu, Yu Zhao, Yangyang Niu, Qionghong Xie, Hao Nie, Yun Jin, Yingying Zhang, Yuqiu Lu, Saiya Zhu, Wei Zuo, et al.(Shanghai) Company Ltd], CD68 [GM081402, 1:200, Gene Tech (Shanghai) Company Ltd] and S100A8/A9 complex (ab22506, 1:1000, Abcam). The positive cells for CD3, CD20, CD68 and S100A8/A9 staining were counted under a

(2) The Cxcr2+ subset of the S100a8+ gastric granylocytic myeloid-derived suppressor cell population (G-MDSC) regulates gastric pathologyFrontiers in ImmunologySeptember 8, 2023Krystal D. Kao, Helmut Grasberger, Mohamad El-Zaatariperformed as described previously (10), using antibodies against the following targets: calprotectin (#ab22506, Abcam); CD11b-AF594 (clone M1/70, #101254, BioLegend); Cxcr2-AF488 (#FAB2164G, R&D Systems); GSII-FITC

(3) Molecular Magnetic Resonance Imaging of Aneurysmal Inflammation Using a Redox Active Iron ComplexInvestigative RadiologySeptember 1, 2023Robert M. King, Matthew J. Gounis, Eric J. Schmidt, Anita Leporati, Eric M. Gale, Alexei A. Bogdanovserum in TBS, pH 7.5 for 30 min. For IHC detection of calprotectin anti-(S100A8/A9 complex)/S100A9 (ab22506 Abcam, Waltham MA) antibody was covalently labeled by using digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester

(4) Organic Matrix Derived from Host–Microbe Interplay Contributes to Pathological Renal BiomineralizationACS Nanoscience AuAugust 16, 2023Yushi Bai, Yongmei Wang, Misun Kang, Claire M. Gabe, Sudarshan Srirangapatanam, Austin Edwards, Marshall Stoller, Stefan J. Green, Shaul Aloni, Nobumichi Tamura, et al.anti-myeloperoxidase (rabbit monoclonal, ab208670, Abcam, Cambridge), and anti-calprotectin (mouse monoclonal, ab22506, Abcam, Cambridge) were incubated on the sections under static conditions overnight at 4 °C. The sections

(5) Phosphorylcholine and KR12-Containing Corneal Implants in HSV-1-Infected Rabbit CorneasPharmaceuticsJune 5, 2023Kamal Malhotra, Oleksiy Buznyk, Mohammad Mirazul Islam, Elle Edin, Sankar Basu, Marc Groleau, Delali Shana Dégué, Per Fagerholm, Adrien Fois, Sylvie Lesage, et al.UK",1:50 lymphatic vessels,"Anti-Lyve 1, ab14917, Abcam, Cambridge, UK",1:100 Macrophages,"Anti-MAC 387, ab22506, Abcam, Cambridge, UK",1:100 Nerves,"neuron-specific Anti-beta III Tubulin [Tu-20], ab7751, Abcam,

(6) Fibroblast Growth Factor 2 Is Produced By Renal Tubular Cells to Act as a Paracrine Factor in Maladaptive Kidney Repair After Cisplatin NephrotoxicityLaboratory investigation; a journal of technical methods and pathologyMarch 1, 2023Hu X, Ma Z, Li S, Wen L, Huo Y, Wu G, Manicassamy S, Dong Z.(SK-5105; Vector Laboratories). The primary antibodies for immunostaining included antimacrophage (ab22506, Abcam) and antie-α-SMA (ab5694, Abcam). Sirius red staining was performed according to a standard protocol

(7) Comparative lipid profiling of murine and human atherosclerotic plaques using high-resolution MALDI MSIPflügers Archiv - European Journal of PhysiologyFebruary 1, 2022Pegah Khamehgir-Silz, Stefanie Gerbig, Nadine Volk, Sabine Schulz, Bernhard Spengler, Markus Hecker, Andreas H. Wagnerstained according to standard procedures for MAC387 immunopositive infiltrating macrophages (1:100, ab22506, Abcam, UK) and CD31 (1:100, M0823, Agilent Dako, USA) using donkey-anti-mouse cy3 [715-166-020] and

(8) Dexamethasone modulates immature neutrophils and interferon programming in severe COVID-19Nature MedicineJanuary 1, 2022Sarthak Sinha et al.with 10% normal donkey serum in PBS (with 0.5% Triton X-100), and primary antibodies (S100A8/9, Abcam, ab22506; IFITM1, Abcam, ab233545) were incubated at 4 °C overnight, followed by incubation with donkey anti-rabbit

(9) Chemokine‐Capturing Wound Contact Layer Rescues Dermal HealingAdvanced ScienceSeptember 1, 2021Lucas Schirmer, Passant Atallah, Uwe Freudenberg, Carsten Wernerfollowed by primary antibody incubation in TBST (Sigma Aldrich) for 2 h. Mouse anti‐S100A9 (1:100, Abcam, ab22506) was used to evaluate inflammatory neutrophil and macrophage presence. After washing three times in

(10) Transcriptional profiling of pediatric cholestatic livers identifies three distinct macrophage populationsPLOS ONEJanuary 7, 2021Sarah A. Taylor et al.were stained for CD68 (Abcam ab955), CD69 (Abcam ab233396), C1Q (Abcam ab268120), and S100A8/9 (Abcam ab22506). Whole slide fluorescent imaging was performed followed by multispectral imaging of three 2.01 mm x

(11) Interleukin-17A pathway target genes are upregulated in Equus caballus supporting limb laminitisPLOS ONEDecember 10, 2020Lynne Cassimeris, Julie B. Engiles, Hannah Galantino-Homersections. Immunofluorescence Tissue sections were also used to localize calprotectin using mouse antibody ab22506 (1:5000; Abcam) recognizing S100A9, followed by goat anti mouse IgG-Alexa 488 (1:100), based on a protocol

(12) Prognostic Role of Survivin and Macrophage Infiltration Quantified on Protein and mRNA Level in Molecular Subtypes Determined by RT-qPCR of KRT5, KRT20, and ERBB2 in Muscle-Invasive Bladder Cancer Treated by Adjuvant ChemotherapyInternational Journal of Molecular SciencesOctober 8, 2020Thorsten H. Ecke et al.(concentration 1:5; ab845, Abcam, U.K.) and mouse monoclonal IgG1 antiMAC387 (concentration 1:500; ab22506, Abcam, U.K.), which detects the myelomonocytic L1 molecule calprotectin. CLEVER-1 (common lymphatic

(13) Iron imaging in myocardial infarction reperfusion injuryNature communicationsJune 29, 2020Moon BF, Iyer SK, Hwuang E, Solomon MP, Hall AT, Kumar R, Josselyn NJ, Higbee-Dempsey EM, Tsourkas A, Imai A, et al.LS‑C755633, 1:100 dilution), Cathepsin-S antibody (GeneTex: GTX114350) and a MAC-387 antibody (Abcam: ab22506), respectively. Tissue fiducial markers, RV insertion, papillary muscle shape, length of the LV, and

(14) A Metabolic Intravascular Platform to Study FDG Uptake in Vascular InjuryCardiovascular Engineering and TechnologyJune 1, 2020F. Franchi, M. Olthoff, J. Krier, C. Noble, M. Al-Hijji, V. Ramaswamy, T. Witt, M. Burke, M. Benscoter, A. Lerman, et al.Additional unstained slides were used for immunohistochemistry staining for MAC387 (macrophage marker, Abcam ab22506, 1:50 overnight at 4°C), VCAM-1 (vascular cell adhesion molecule-1, Abcam ab98954, 8 μg/ml overnight

(15) Obeticholic Acid Ameliorates Valproic Acid–Induced Hepatic Steatosis and Oxidative StressMolecular PharmacologyMay 1, 2020Zhibo Gai, Evelin Krajnc, Sophia L. Samodelov, Michele Visentin, Gerd A. Kullak-Ublickmicrowave-based antigen-retrieval technique. The antibodies used in this study were against the macrophage marker MAC387 (ab22506; Abcam), 4hydroxynonenal (4-HNE) (ab46545; Abcam), and the endoplasmic reticulum chaperone protein Grp78

(16) Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathologyNature protocolsMay 1, 2020Bucur O, Fu F, Calderon M, Mylvaganam GH, Ly NL, Day J, Watkin S, Walker BD, Boyden ES, Zhao Y.,Agilent Technologies,M085129-2,AB_2811108 IgD,Goat,poly,SouthernBiotech,2030-01,AB_2795623 S100A8,Mouse,mono,Abcam,ab22506,AB_447111 MCT1,Chicken,poly,Sigma Aldrich,AB1286-I,AB_11212410 Tom20,Rabbit,poly,Sigma Aldrich,HPA011562,AB_1080326 Nephrin,Rabbit,poly,Santa

(17) Mfsd2a and Spns2 are essential for sphingosine-1-phosphate transport in the formation and maintenance of the blood-brain barrierScience advancesMay 1, 2020Wang Z, Zheng Y, Wang F, Zhong J, Zhao T, Xie Q, Zhu T, Ma F, Tang Q, Zhou B, et al.PA5-28584, Invitrogen), S100A8 (rabbit, 1:100; ab92331, Abcam), S100A9/calprotectin (mouse, 1:200; ab22506, Abcam), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (rabbit, 1:1000; ab181602, Abcam), Mfsd2a

(18) Effects of Farnesiferol B on Ischemia-Reperfusion-Induced Renal Damage, Inflammation, and NF-κB SignalingInternational Journal of Molecular SciencesDecember 12, 2019Lu Zhang, Xianjun Fu, Ting Gui, Tianqi Wang, Zhenguo Wang, Gerd A. Kullak-Ublick, Zhibo Gailipocalin (NGAL, ab63929, Abcam), Ly-6B (NBP2-13077), NFκB p65 (sc-372, Santa Cruz, CA, USA), and MAC387 (ab22506, Abcam). Sections were treated with the Envision+ DAB kit (Dako, Basel, Switzerland) according to the

(19) Immunohistochemical characterisation of the hepatic stem cell niche in feline hepatic lipidosis: a preliminary morphological studyJournal of Feline Medicine and SurgeryFebruary 1, 2019Chiara Valtolina, Joris H Robben, Robert P Favier, Jan Rothuizen, Guy CM Grinwis, Baukje A Schotanus, Louis C Penningcode,Dilution K19,Mouse,IgG1,b170,Prot K,O/N 4°C,Novocastra,NCL-CK19,1:100 MAC387,Mouse,IgG1,MAC387,Prot K,O/N 4°C,Abcam,ab22506,1:1000 α-SMA,Mouse,IgG2a,1A4,None,60 mins RT,BioGenex,MU128-UC,1:200 LAM,Rabbit,Ig,,Prot K,O/N 4°C,Abcam,ab11575,1:100 Ki67,Mouse,IgG1,MIB1,Citrate

(20) High-resolution Imaging of Myeloperoxidase Activity Sensors in Human Cerebrovascular DiseaseScientific ReportsDecember 1, 2018Youssef Z. Wadghiri, Dung Minh Hoang, Anita Leporati, Matthew J. Gounis, Aurora Rodríguez-Rodríguez, Mary L. Mazzanti, John P. Weaver, Ajay K. Wakhloo, Peter Caravan, Alexei A. Bogdanovgranulocytes, monocytes and macrophages) were detected by using anti-calprotectin (S100A8/A9 complex) antibody (ab22506, Abcam); ND- not done.

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Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] from Abcam

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Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] from Abcam

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