Anti-HLA Class I antibody [W6/32] - Low endotoxin, Azide free ab23755 from Abcam

Product Specs
ItemAnti-HLA Class I antibody [W6/32] - Low endotoxin, Azide free
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab23755
Antibody TypePrimary
CloneW6/32
Quantity100 UG
ClonalityMonoclonal
IsotypeIgG2a
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence
HostMouse, Nude mouse
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameHLA-A
ReactivityHuman
TargetHLA Class I

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Immune desert in MMR-deficient tumors predicts poor responsiveness of immune checkpoint inhibitionFrontiers in ImmunologyApril 28, 2023Guoxing Zheng, Yingsi Lu, Zheng Yang, Hong Chen, Qian Liang, Qingqing Zhu, Yan Li, Xing Xiao, Zhuzhen He, Yifan Zhu, et al.(Ref# ab4055), PD-1 (Ref# ab52587), PD-L1 (Ref# ab213524), and human leukocyte antigen (HLA) I (Ref# ab23755), respectively, and counterstained with hematoxylin. The antibody-specific staining on the slides was

(2) A chemical screen underscores the essential role of STAT1-dependent IFNγ signaling to regulate HLA-I expression in cancer cellsMicroPubl BiolJanuary 18, 2023Myriam Barz, Bartlomiej Porebski, Pranauti Panshikar, Maria Häggbladd, Daniela Hühn, Oscar Fernandez-Capetillohours. Cells were fixed with 4% formaldehyde and washed with PBS. The Anti-HLA Class I antibody [W6/32] (ab23755, Abcam) (final 1:2000 in 3% BSA and 0.1% Tween) was added on top and incubated at 4°C overnight. Next

(3) Increased expression of cell adhesion molecules in myofasciitisFrontiers in neurologyJanuary 1, 2023Ma X, Gao H, Xu L, Bi Z, Ji S, Bu B.immunofluorescence staining, expression of major histocompatibility complex class I (MHC-I) (1:100, ab23755, Abcam) and deposition of membrane attack complex (MAC) (1:50, Sc-58,935, Santa Cruz Biotechnology)

(4) Modelling acute antibody-mediated rejection of human kidney transplants using ex-vivo warm machine perfusioneBioMedicineDecember 1, 2022Pankaj Chandak, Benedict L. Phillips, Danothy Bennett, Raphael Uwechue, Nicos Kessaris, Olivia Shaw, Tim Maggs, Luke Woodford, David Veniard, Ranmith Perera, et al.antibody for the HLAi AMR model, we used commercially available mouse anti-human class I monoclonal W6/32 (ab23755 Mouse monoclonal [W6/32]) to HLA Class I with low endotoxin and Azide free (Abcam Company, UK, RRID:

(5) The Clinicopathological Distinction Between Seropositive and Seronegative Immune-Mediated Necrotizing Myopathy in ChinaFrontiers in neurologyJanuary 1, 2021Ma X, Xu L, Ji S, Li Y, Bu B.(1:200, PB9050, Boster). The expression of major histocompatibility complex class I (MHC-I) (1:100, ab23755, Abcam) on the sarcolemma, deposition of membrane attack complex (MAC) (1:50, Sc-58935, Santa Cruz Biotechnology)

(6) HIV Reactivation in Latently Infected Cells with Virological Synapse-Like Cell ContactVirusesApril 8, 2020Toshiki Okutomi, Satoko Minakawa, Riku Hirota, Koko Katagiri, Yuko MorikawaBiotechnology, Dallas, TX, USA), anti-CD3ε mouse mAb (HIT3a, BD Pharmingen), and anti-HLA class I mouse mAb (ab23755, Abcam). Alexa Fluor 488-conjugated or Alexa Fluor 568-conjugated anti-mouse, Alexa Fluor 568-conjugated

(7) Two Functional Variants of AP-1 Complexes Composed of either γ2 or γ1 Subunits Are Independently Required for Major Histocompatibility Complex Class I Downregulation by HIV-1 NefJournal of virologyMarch 17, 2020Tavares LA, de Carvalho JV, Costa CS, Silveira RM, de Carvalho AN, Donadi EA, daSilva LLP.antibody, a mouse anti-HLA class I antibody that recognises HLA-A, -B, and -C loci (W6/32, catalog no. ab23755; Abcam, Cambridge, MA) was used. Rabbit polyclonal anti-HRS (catalog no. ab155539; Abcam, Cambridge,

(8) Enrichment of CD146⁺ Adipose-Derived Stem Cells in Combination with Articular Cartilage Extracellular Matrix Scaffold Promotes Cartilage RegenerationTheranosticsJanuary 1, 2019Li X, Guo W, Zha K, Jing X, Wang M, Zhang Y, Hao C, Gao S, Chen M, Yuan Z, et al.0.25% Triton X-100 in PBS for 20 min. Next, cells were incubated with anti-HLA Class I (Abcam, ref. ab23755) and anti-MHC-II (Abcam, ref. ab157210) at 4°C overnight. The immunodetection was performed using DAB

(9) IFN-t Mediated Control of Bovine Major Histocompatibility Complex Class I Expression and Function via the Regulation of bta-miR-148b/152 in Bovine Endometrial Epithelial CellsFrontiers in ImmunologyFebruary 2, 2018Haichong Wu, Kangfeng Jiang, Shuai Guo, Jing Yang, Gan Zhao, Changwei Qiu, Ganzhen Deng

(10) Interferon-τ increases BoLA-I for implantation during early pregnancy in dairy cowsOncotargetNovember 10, 2017Zhe Zhu, Binbin Li, Yue Wu, Xiao Wang, GanZhen Dengp-STAT1 and p-STAT3 were purchased from Cell Signaling Technology Inc (Beverly, MA, USA). HLA class I (ab23755, reacts with cow BoLA-I), and horseradish peroxidase conjugated goat anti-rabbit antibodies were provided

(11) Targeting sarcoma tumor-initiating cells through differentiation therapyStem Cell ResearchMay 1, 2017Dan Han, Veronica Rodriguez-Bravo, Elizabeth Charytonowicz, Elizabeth Demicco, Josep Domingo-Domenech, Robert G. Maki, Carlos Cordon-Cardoperformed on cell lines and FFPE tissue sections. Primary antibodies for HLA class I W6/32 (Abcam, ab23755), CD44 (BD, 550392), Sox-9 (Santa Cruz, sc2009), and actin (Sigma, AC-15) were used. 2.4 Tumor Xenograft

(12) Quantitative Analysis of Cellular Proteome Alterations in CDV-Infected Mink Lung Epithelial CellsFrontiers in microbiologyJanuary 1, 2017Tong M, Yi L, Sun N, Cheng Y, Cao Z, Wang J, Li S, Lin P, Sun Y, Cheng S.(IκB-β) rabbit polyclonal antibody (PA5-40909, ThermoFisher, U.S.A), MHC-I mouse monoclonal antibody (ab23755, Abcam, UK), RPS29 rabbit polyclonal antibody (PA5-41744, ThermoFisher, U.S.A), IκB-α rabbit polyclonal

(13) Implantation of Stromal Vascular Fraction Progenitors at Bone Fracture Sites: From a Rat Model to a First-in-Man StudyStem cells (Dayton, Ohio)December 1, 2016Franziska Saxer, Arnaud Scherberich, Atanas Todorov, Patrick Studer, Sylvie Miot, Simone Schreiner, Sinan Güven, Laurent A H Tchang, Martin Haug, Michael Heberer, Dirk J Schaefer, Daniel Rikli, Ivan Martin, Marcel Jakob

(14) An oncofetal antigen, IMP-3-derived long peptides induce immune responses of both helper T cells and CTLsOncoimmunologyJune 1, 2016Hirayama M, Tomita Y, Yuno A, Tsukamoto H, Senju S, Imamura Y, Sayem MA, Irie A, Yoshitake Y, Fukuma D, et al.HLA-DR (BioLegend, 307612), HLA-DP (Abcam, ab106312), HLA-DQ (Abcam, ab23632), or HLA-class I (Abcam, ab23755) were added into the culture. All mAbs were used at 5 μg/mL. Cytokine analysis IMP-3-LP-specific bulk

(15) IGKV3 proteins as candidate "off-the-shelf" vaccines for kappa-light chain-restricted B-cell non-Hodgkin lymphomasClinical cancer research : an official journal of the American Association for Cancer ResearchAugust 1, 2012Debora Martorelli, Massimo Guidoboni, Valli De Re, Elena Muraro, Riccardo Turrini, Anna Merlo, Elisa Pasini, Laura Caggiari, Luca Romagnoli, Michele Spina, Roberta Mortarini, Daniela Gasparotto, Mario Mazzucato, Antonino Carbone, Antonio Rosato, Andrea Anichini, Riccardo Dolcetti

(16) The HCMV membrane glycoprotein US10 selectively targets HLA-G for degradationJournal of Experimental MedicineAugust 30, 2010Boyoun Park, Eric Spooner, Brandy L. Houser, Jack L. Strominger, Hidde L. Ploegh

(17) Cell-based immunotherapy with suppressor CD8+ T cells in rheumatoid arthritisJournal of immunology (Baltimore, Md. : 1950)June 1, 2005Eduardo Davila, Young Mo Kang, Yong Wook Park, Hirokazu Sawai, Xiaowen He, Sergey Pryshchep, Jörg J Goronzy, Cornelia M Weyand

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Anti-HLA Class I antibody [W6/32] - Low endotoxin, Azide free from Abcam

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Anti-HLA Class I antibody [W6/32] - Low endotoxin, Azide free from Abcam

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