Fig 1: Cell proliferation was decreased in hNPCs exposed to high glucose. (a) Representative images of hNPCs expressing neural stem cell markers i.e., Nestin (i) SOX2 (ii), and Musashi1 (iii). Nuclei are stained with DAPI (blue). (b) There was significant decrease in cell proliferation index in hNPCs exposed to high glucose as depicted by MTS assay in n = 4, ***p < 0.001. (c) Western blot analysis revealed decrease expression of Nestin and Musashi1 in hNPCs exposed to HG for 96 h. (d) Quantitative analysis shows significant decrease in Nestin (Observed band size 300 kDa, Cat. no. ab18102, Abcam) and Musashi1 (39 kDa) protein expression in hNPCs exposed to HG for 96 h. Data is represented as Mean ± SE, n = 4, *p < 0.05, **p < 0.01. Uncropped blot images are shown in Supplementary Fig. S2.