His-Tag (27E8) Mouse mAb 2366S from Cell Signaling Technology

Product Specs
ItemHis-Tag (27E8) Mouse mAb
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number2366S
Quantity100 µl
ApplicationsW, W-S, IP
ReactivityAll
TargetHis-Tag
HostMouse
ClonalityMonoclonal
Research AreaRelated Products
Clone2700000000

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) A planar polarized MYO6-DOCK7-RAC1 axis promotes tissue fluidification in mammary epitheliaCell ReportsAugust 16, 2023Luca Menin, Janine Weber, Stefano Villa, Emanuele Martini, Elena Maspero, Carlos A. Niño, Valeria Cancila, Alessandro Poli, Paolo Maiuri, Andrea Palamidessi, et al.Cruz,Cat#166600 anti-CDC42,Santa Cruz,Cat#390210 anti-GFP,Sigma-Aldrich,Cat#G1544 anti-His,Cell Signaling,Cat#2366 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(2) EPLIN-β is a novel substrate of ornithine decarboxylase antizyme 1 and mediates cellular migrationJournal of Cell ScienceJune 15, 2023Dan Li, Suat Peng Neo, Jayantha Gunaratne, Kanaga Sabapathyanti-rabbit secondary antibody (7074S, lot 29), anti-HA-tag (C29F4) (3724S, lot 10) and anti-His-tag (27E8) (2366S, lot 14) from Cell Signaling Technology, Danvers, MA, USA. The antibody dilution factors are shown in

(3) Hepatic nonvesicular cholesterol transport is critical for systemic lipid homeostasisNature MetabolismJanuary 16, 2023Xu Xiao, John Paul Kennelly, Alessandra Ferrari, Bethan L. Clifford, Emily Whang, Yajing Gao, Kevin Qian, Jaspreet Sandhu, Kelsey E. Jarrett, Madelaine C. Brearley-Sholto, et al.washed with DPBS three times. Cells were incubated with an anti-His antibody (27E8, Cell Signaling, 2366S; diluted 1:1,000) overnight before incubation with fluorescently labeled secondary antibodies for 1

(4) Molecular mechanism of Afadin substrate recruitment to the receptor phosphatase PTPRK via its pseudophosphatase domaineLifeOctober 20, 2022Iain M Hay, Katie E Mulholland, Tiffany Lai, Stephen C Graham, Hayley J Sharpe, Janet E Deane,Cell Signaling Technology,Cat#13531,Western blot: 1:1000 Antibody,Anti-His (mouse monoclonal),Cell Signaling Technology,Cat#2366,Western blot: 1:1000 Antibody,Anti-phospho-tyrosine (P-Tyr-1000) (rabbit monoclonal),Cell Signaling Technology

(5) USP13 promotes deubiquitination of ZHX2 and tumorigenesis in kidney cancerProceedings of the National Academy of Sciences of the United States of AmericaSeptember 6, 2022Xie H, Zhou J, Liu X, Xu Y, Hepperla AJ, Simon JM, Wang T, Yao H, Liao C, Baldwin AS, et al.Rabbit anti-HA-tag (3724), rabbit anti-FLAG-tag (14793), rabbit-anti VHL (68547), mouse anti-His-tag (2366), and mouse anti-α-tubulin (3873S) were from Cell Signaling Technology. Mouse anti-GAPDH (TA-08) was

(6) Perinatal versus adult loss of ULK1 and ULK2 distinctly influences cardiac autophagy and functionAutophagySeptember 1, 2022Harris MP, Zhang QJ, Cochran CT, Ponce J, Alexander S, Kronemberger A, Fuqua JD, Zhang Y, Fattal R, Harper T, et al.UBB, UBC and RPS27A; 1:1000; 3936), BECN1 (1:1000; 3738), BECN1 S30 (1:1000; 54101), HIS-TAG (1:1000; 2366), and BECN1 S15 (1:1000; 84966) from Cell Signaling Technologies, GAPDH (1:2000; sc-32233), NBR1 (1:1000;

(7) Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seqCellJuly 7, 2022Joseph M. Replogle et al.RRID: AB_11125549 α-INTS14,Prestige,Cat# HPA040651 α-C7orf26,Prestige,Cat# HPA052175 α-His,CST,Cat# 2366 α-Mouse,Licor,Cat# 926-32210 α-Rabbit,Licor,Cat# 926-32213 α-CD11b AF647,Biolegend,Cat# 101220; RRID:

(8) p21-activated kinase 4 controls the aggregation of α-synuclein by reducing the monomeric and aggregated forms of α-synuclein: involvement of the E3 ubiquitin ligase NEDD4-1Cell Death & DiseaseJune 30, 2022So-Yoon Won, Jung-Jin Park, Soon-Tae You, Jong-A Hyeun, Hyong-Kyu Kim, Byung Kwan Jin, Catriona McLean, Eun-Young Shin, Eung-Gook Kim(sc8334, Santa Cruz Biotechnology), rabbit anti-Myc (sc789, Santa Cruz Biotechnology), rabbit anti-His (#2366S, Cell Signaling), and GST-conjugated horseradish peroxidase (HRP) (ab3416, Abcam), tamoxifen (T5648,

(9) A chemoproteoinformatics approach demonstrates that aspirin increases sensitivity to MEK inhibition by directly binding to RPS5PNAS NexusJune 15, 2022Motoki Watanabe, Shogen Boku, Kaito Kobayashi, Yoichi Kurumida, Mamiko Sukeno, Mitsuharu Masuda, Katsura Mizushima, Chikage Kato, Yosuke Iizumi, Kiichi Hirota, et al.(#9542), rabbit anti-Puma (#4976), rabbit anti-p53 (#9282), mouse anti-His-Tag monoclonal antibody (#2366; Cell Signaling Technology, Beverly, MA, USA), rabbit anti-BIM (ab32158), rabbit anti-RPS5 (ab58345,

(10) Angiopoietin-2 Inhibition of Thrombomodulin-Mediated Anticoagulation-A Novel Mechanism That May Contribute to Hypercoagulation in Critically Ill COVID-19 PatientsBiomedicinesJune 6, 2022Hultström M, Fromell K, Larsson A, Persson B, Nilsson B, Quaggin SE, Betsholtz C, Frithiof R, Lipcsey M, Jeansson M.were blocked with 5% BSA for 1 h and incubated overnight with mouse anti-6X His-tag antibody (27E8, 2366, Cell Signaling, Danvers, MA, USA). After washing and incubating with anti-mouse IgG-HRP-conjugated

(11) Development of a colorimetric assay for the detection of SARS-CoV-2 3CLpro activityBiochemical JournalApril 29, 2022Gavin D. Garland, Robert F. Harvey, Thomas E. Mulroney, Mie Monti, Stewart Fuller, Richard Haigh, Pehuén Pereyra Pereyra Gerber, Michael R. Barer, Nicholas J. Matheson, Anne E. Willisbleed" Anti-SARS-CoV-2 Spike,Mouse,1 : 5000,GeneTex,632604 Anti-His-Tag,Mouse,1 : 1000,Cell signalling technologies,2366 Anti-His-Tag,Rabbit,1 : 1000,Cell signalling technologies,2365 Anti-β-Tubulin (TUBB),Rabbit,1 : 1000,Cell signalling

(12) Identifying Small-Molecule Inhibitors of SARS-CoV-2 RNA-Dependent RNA Polymerase by Establishing a Fluorometric AssayFrontiers in ImmunologyApril 7, 2022Xiaoming Bai, Hongmin Sun, Shuo Wu, Yuhuan Li, Lifei Wang, Bin Hong120 min at room temperature, rinsed with TBST, and stained with antibodies against His tag (Cat no. 2366S, Lot no. 15, Cell Signaling Technology, Inc., Danvers, MA, USA) or GST tag (Cat no. 2368T, Lot no. 15,

(13) Development of an Oral Salmonella-Based Vaccine Platform against SARS-CoV-2Vaccines (Basel)January 1, 2022Wonsuck Yoon, Yongsung Park, Seunghyun Kim, Iel Soo Bangmilk overnight and were incubated with mouse anti-His antibody (Cell Signaling Technology, Catalog #2366) for 2 h. The membranes were incubated with goat anti-mouse IgG HRP conjugate for 40 min at room temperature.

(14) Characterization of recombinant β subunit of human MUC4 mucin (rMUC4β)Scientific ReportsDecember 9, 2021Prakash G. Kshirsagar et al.Bacto yeast, and Bacto Agar were purchased from BD Biosciences. Anti-His tag Ab (Clone 27E9, Cat. No. 2366) and anti-EGFR mAb (Clone D38B1, Cat. No. 4267) were purchased from Cell Signaling Technology, while

(15) Structural studies of phosphorylation-dependent interactions between the V2R receptor and arrestin-2Nature CommunicationsDecember 1, 2021Qing-Tao He et al.anti-GST (Cell Signaling Technology, Catalog #2622) and anti-His (Cell Signaling Technology, Catalog #2366) were used in 1:1000 dilution. The secondary antibodies including anti-rabbit (Sigma Aldrich, Catalog

(16) DNA polymerase zeta contributes to heterochromatin replication to prevent genome instabilityThe EMBO journalNovember 2, 2021Ben Yamin B, Ahmed-Seghir S, Tomida J, Despras E, Pouvelle C, Yurchenko A, Goulas J, Corre R, Delacour Q, Droin N, et al.anti‐HA Tag,Cell Signaling Technologies,3724 Mouse monoclonal anti‐His,Cell Signaling Technologies,2366 Mouse monoclonal anti‐V5,Invitrogen,R962‐25 Mouse monoclonal anti‐HP1γ,Millipore Sigma,MAB3450 Anti‐GST‐HRP

(17) Feedback control of PLK1 by Apolo1 ensures accurate chromosome segregationCell reportsJuly 13, 2021Xu L, Ali M, Duan W, Yuan X, Garba F, Mullen M, Sun B, Poser I, Duan H, Lu J, et al.,Cell Signaling Technology,Cat#2624; RRID:AB_2189875 Mouse monoclonal anti-His,Cell Signaling Technology,Cat#2366; RRID:AB_2115719 Rabbit monoclonal anti-GFP,Cell Signaling Technology,Cat#2037; RRID:AB_1281301 Mouse

(18) Crystal structure of human PACRG in complex with MEIG1 reveals roles in axoneme formation and tubulin bindingStructure (London, England : 1993)June 3, 2021Khan N, Pelletier D, McAlear TS, Croteau N, Veyron S, Bayne AN, Black C, Ichikawa M, Khalifa AAZ, Chaaban S, et al.Santa Cruz Biotechnology (#sc-373851). The His-tag polyclonal rabbit antibody was from Cell Signaling Technology (#2366P). Protein expression and purification Purification of His6-MEIG1:PACRGFL, His6-MEIG1, and His6-PACRGL. BL21

(19) ATPase and helicase activities of porcine epidemic diarrhea virus nsp13Veterinary MicrobiologyJune 1, 2021Jie Ren, Zhen Ding, Puxian Fang, Shaobo Xiao, Liurong Fangwith 5 % skim milk and immunoblotted with an anti-His monoclonal antibody (Cell Signaling Technology, #2366). 2.5 ATPase activity assay The Kinase-Glo Plus Luminescent Kinase Assay kit (Promega, USA) was used

(20) Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesisCell metabolismMay 4, 2021Wang YP, Sharda A, Xu SN, van Gastel N, Man CH, Choi U, Leong WZ, Li X, Scadden DT.anti-MT-CO2,Proteintech,Cat#: 55070-1-AP Mouse monoclonal anti-His (clone 27E8),Cell Signaling Technology,Cat# 2366; RRID: AB_2115719 Rabbit monoclonal anti-TOM20 (clone 29),BD Biosciences,Cat#612278; RRID: AB_399595 Rabbit

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5/12/2022Good Antibody to Detect His-Tagged Proteins Yashavantha VishweshwaraiahWe used this antibody to detect E. coli expressed N-terminal His-tagged recombinant proteins. The antibody wors well. It shows reasonably good specificity.

His-Tag (27E8) Mouse mAb

His-Tag (27E8) Mouse mAb from Cell Signaling Technology

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Cell Signaling Technology

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His-Tag (27E8) Mouse mAb from Cell Signaling Technology

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