Fig 2: Celigo Image Cytometer analysis shows that knocking down OIP5 significantly inhibits U251 cell proliferation. (A) and (B) The number of cells in each colony was counted over 5 days. (C) and (D) Cell number in the OIP5-siRNA group significantly decreased from days 3 to 5 compared to the control group (P < 0.05).

Fig 3: Knockdown of OIP5 induces cell cycle arrest in U251 cells. The effect of silencing of OIP5 on the cell cycle was evaluated by flow cytometric assay. A significant increase in the cell population in the G1 and G2 phase and a significant decrease of the cell population in the S phase were detected in the OIP5-siRNA group compared with control U251 cells (*P < 0.05, **P < 0.01).

Fig 4: OIP5 expression in glioma cell lines and OIP5–siRNA inhibition of OIP5 expression in cells. (A): RT-PCR revealed that the OIP5 gene is abundantly or moderately expressed in U251, U373, A172, and glioblastoma of unknown origin cell lines. (B) and (C): Effect of OIP5 knockdown on the levels of OIP5 protein in 293 T cells as detected by western blot analysis. D-G: Analysis of GFP expression in U251 cells after infection with a lentiviral vector expressing GFP. (D) and (E): U251 cells in the control group (×200). (F) and (G): GFP expression in the OIP5-siRNA group (×200), reflecting infection efficiency in U251 cells. (H) Infection with Lentivirus-siOIP5 inhibited OIP5 mRNA expression in U251 cells as determined by RT-PCR. (**P < 0.05).

Fig 5: MTT assay showed that OIP5 knockdown inhibits U251 cell proliferation. Infection with lentivirus-siOIP5 significantly inhibited cell proliferation in U251 cells from day 3 to day 5. Cell counts were determined by MTT assay over 5 days (P < 0.05).