Anti-Phospho - (Ser/Thr) Phe antibody ab17464 from Abcam

Product Specs
ItemAnti-Phospho - (Ser/Thr) Phe antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab17464
Antibody TypePrimary
ClonalityPolyclonal
ApplicationsELISA, Immunoprecipitation, Western blot
HostRabbit
IsotypeIgG
Quantity50 UL
Conjugate/TagUnconjugated
Concentration0.075 mg/ml
ReactivitySpecies independent
TargetPhospho - (Ser/Thr) Phe

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) SAD-1 kinase controls presynaptic phase separation by relieving SYD-2/Liprin-α autoinhibitionPLoS BiolDecember 4, 2023Nathan A. McDonald, Li Tao, Meng-Qiu Dong, Kang Shenand blotted for GFP (Roche 11814460001), mScarlet (ChromoTek 6G6), or phospho-serine/threonine (Abcam ab17464). Purified protein constructs were produced in Rosetta2(DE3) E. coli cells grown at 37°C in TB medium

(2) PWL1, a G‐type lectin receptor‐like kinase, positively regulates leaf senescence and heat tolerance but negatively regulates resistance to Xanthomonas oryzae in ricePlant Biotechnology JournalDecember 1, 2023Jiangmin Xu, Chunlian Wang, Fujun Wang, Yapei Liu, Man Li, Hongjie Wang, Yuhan Zheng, Kaijun Zhao, Zhiyuan Jiof PWL1 was evaluated by immunoblotting analysis using an antiphospho (Ser/Thr) Phe antibody (Abcam, ab17464). Protein interaction assay For the bimolecular fluorescence complementation (BiFC) assay, the full‐length

(3) Dynamic Phosphorylation of G9a Regulates its Repressive Activity on Chromatin Accessibility and Mitotic ProgressionAdvanced ScienceOctober 1, 2023Qizhi Geng, Yue‐Yu Kong, Weizhe Li, Jianhao Zhang, Haoli Ma, Yuhang Zhang, Lin‐Tai Da, Yan Zhao, Hai‐Ning Dua‐cyclin B1 (1495‐1), a‐histone H3 pS10 (1173‐1) were purchased from Epigenomics; a‐pan‐phosoho‐(Ser/Thr) (ab17464), a‐H3K9me1(ab8896), a‐H3K9me2 (ab1220), and a‐H3K9me3 (ab8898) antibodies were purchased from Abcam;

(4) SAD-1 kinase controls presynaptic phase separation by relieving SYD-2/Liprin-α autoinhibitionbioRxivJune 17, 2023Nathan A. McDonald, Li Tao, Meng-Qiu Dong, Kang Shenand blotted for GFP (Roche 11814460001), mScarlet (ChromoTek 6G6), or phospho-serine/threonine (Abcam ab17464). C. elegans were prepared for phosphoproteomics as described previously39. 14N sad-1Δ and 15N-labeled

(5) STK33 Phosphorylates Fibrous Sheath Protein AKAP3/4 to Regulate Sperm Flagella Assembly in SpermiogenesisMolecular & cellular proteomics : MCPJune 1, 2023Yu W, Li Y, Chen H, Cui Y, Situ C, Yao L, Zhang X, Lu S, Liu L, Li L, et al.E-AB-65117), anti-GAPDH (Abways, AB0036), anti-His (Abways, AB0002), and anti-Phosph-(Ser/Thr) Phe (Abcam, ab17464). Results Identification of STK33 Gene Mutations in NOA Patients STK33 (NM_030906) is localized

(6) Spatiotemporal formation of the large vacuole regulated by the BIN2-VLG module is required for female gametophyte development in ArabidopsisThe Plant CellMarch 29, 2023Li-Qin Hu, Shi-Xia Yu, Wan-Yue Xu, Song-Hao Zu, Yu-Tong Jiang, Hao-Tian Shi, Yan-Jie Zhang, Hong-Wei Xue, Ying-Xiang Wang, Wen-Hui Linantibody (Abways; cat. no. AB0029; 1:3000 dilution), anti-Phosphor-(Ser/Thr) antibody (Abcam; cat. no. AB17464; 1:4,000 dilution). Immunoblot assay For total protein extraction, equal amounts of 7-day-old seedlings

(7) Role of C-Terminal Phosphorylation of Lamin A in DNA Damage and Cellular SenescenceCellsFebruary 16, 2023Ying Ao, Zhuping Wu, Zhiwei Liao, Juncong Lan, Jie Zhang, Pengfei Sun, Baohua Liu, Zimei WangEMD Millipore (Boston, MA, USA); and anti-KAP-1 (ab10484), anti-p-KAP-1 Ser824 (ab70369), anti-p-S/T (ab17464), anti-GSK3β (ab93926), and anti-GST (ab108524) antibodies were obtained from Abcam (Cambridge, UK).

(8) Orphan Nuclear Receptor Nur77 Mediates the Lethal Endoplasmic Reticulum Stress and Therapeutic Efficacy of Cryptomeridiol in Hepatocellular CarcinomaCellsDecember 1, 2022Li X, Chen Q, Liu J, Lai S, Zhang M, Zhen T, Hu H, Gao X, Wong AST, Zeng JZ.anti-GFP (sc-9996) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); anti-Ser/Thr (ab17464) and anti-IRE1α (phospho S724) (ab124945) were obtained from Abcam. ER stress inhibitor 4-PBA (HY-A0281),

(9) Involvement of adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 in diallyl trisulfide-induced cytotoxicity in hepatocellular carcinoma cellsThe Korean Journal of Physiology & PharmacologyNovember 1, 2022Feng Guan, Youming Ding, Yikang He, Lu Li, Xinyu Yang, Changhua Wang, Mingbai HuThr202/Tyr204 (#4370) antibodies, Cell Signaling Technology (Beverly, MA, USA); anti-phospho -(Ser/Thr) (ab17464), anti-TRAF6 (ab94720), anti-ubiquitin (linkage-specific K63) (ab179434), and anti-ubiquitin (linkage-specific

(10) p21-activated kinase 4 controls the aggregation of α-synuclein by reducing the monomeric and aggregated forms of α-synuclein: involvement of the E3 ubiquitin ligase NEDD4-1Cell Death & DiseaseJune 30, 2022So-Yoon Won, Jung-Jin Park, Soon-Tae You, Jong-A Hyeun, Hyong-Kyu Kim, Byung Kwan Jin, Catriona McLean, Eun-Young Shin, Eung-Gook KimAbcam), mouse anti-phospho-α-synuclein (S129) (SNCA) (ab184674, Abcam), Anti-phospho-serine/threonine (ab17464, Abcam), anti-phospho-tyrosine (05-321, Upstate), rabbit anti-GAPDH (sc25778, Santa Cruz Biotechnology),

(11) Tyrosine phosphorylation tunes chemical and thermal sensitivity of TRPV2 ion channeleLifeJune 10, 2022Mo X, Pang P, Wang Y, Jiang D, Zhang M, Li Y, Wang P, Geng Q, Xie C, Du HN, et al.RRID: AB_828379,WB (1:1000) Antibody,Anti-Phospho-(Ser/Thr)Phe antibody (Rabbit polyclonal),Abcam,Cat#ab17464; RRID: AB_443891,WB (1:1000) Antibody,Anti-Flag antibody (Rabbit polyclonal),Proteintech,Cat#20543–1-AP;

(12) Characterization of Acetylation of Histone H3 at Lysine 9 in the Trigeminal Ganglion of a Rat Trigeminal Neuralgia ModelOxidative Medicine and Cellular LongevityMay 4, 2022Wenbin Wei, Yuemin Liu, Yating Qiu, Minjie Chen, Yiwen Wang, Zixiang Han, Ying ChaiChIP-seq assay. Trigeminal ganglia were harvested and mixed with 1 μg phosphoserine/threonine (Abcam, ab17464), HDAC3 (CST, #85057), LRRK2 (CST, #13046), or IgG Rabbit IgG antibody and 40 μl flurry IgA beads (Thermo Fisher Scientific

(13) AMP-activated protein kinase α1 phosphorylates PHD2 to maintain systemic iron homeostasisClinical and translational medicineMay 1, 2022Wang C, Zhang W, Xu W, Liu Z, Huang K.Cell Signaling Technology, 1:1000), Hydroxy‐HIF1α (Pro564) (3434, Cell Signaling Technology, 1:1000), pSer/Thr (ab17464, Abcam, 1:1000 ), PHD2 (4835, Cell Signaling Technology, 1:1000), HA (3724, Cell Signaling Technology,

(14) RNF115 Inhibits the Post‐ER Trafficking of TLRs and TLRs‐Mediated Immune Responses by Catalyzing K11‐Linked Ubiquitination of RAB1A and RAB13Advanced ScienceMay 1, 2022Zhi‐Dong Zhang, Hong‐Xu Li, Hu Gan, Zhen Tang, Yu‐Yao Guo, Shu‐Qi Yao, Tianzi Liuyu, Bo Zhong, Dandan Linanti‐AKT3 (ABclonal, A12909); anti‐p‐AKT1 (Cell Singling Technologies, 9018S); anti‐p‐Ser/Thr (Abcam, ab17464); anti‐Na/K+ ATPase (Abcam, ab76020). The mammalian expression plasmids for RAB1A, RAB5A, RAB7A, RAB13,

(15) KMT5A-methylated SNIP1 promotes triple-negative breast cancer metastasis by activating YAP signalingNature communicationsApril 21, 2022Yu B, Su J, Shi Q, Liu Q, Ma J, Ru G, Zhang L, Zhang J, Hu X, Tang J.for WB, sc-138) were from Santa Cruz Biotechnology (CA); antibodies for Phospho-Ser/Thr (1:1000 for WB, ab17464), SNIP1 (1:1000 for WB, 1:100 for Co-IP, 4 μg for ChIP, ab126194), acetyl-Histone H3 (Lys9) (4 μg for

(16) Novel role of pre-replication complex component cell division cycle 6 in retinal neovascularizationArteriosclerosis, Thrombosis, and Vascular BiologyApril 1, 2022Raj Kumar, Gadiparthi N. RaoNo. 550274) was bought from BD Pharmingen (Palo Alto, CA). Anti-Ki67 (ab15580), and anti-pSer/Thr (ab17464) antibodies, Fluo-8 calcium flux assay kit (ab112129) and Fluo-8 AM green fluorescent calcium binding dye

(17) Vimentin Inhibits Dengue Virus Type 2 Invasion of the Blood-Brain BarrierFrontiers in Cellular and Infection MicrobiologyMarch 30, 2022Jianhai Yu et al.-polyacrylamide gel electrophoresis (SDS-PAGE), incubated with primary antibody (Anti-Phospho-(Ser/Thr) Phe antibody, ab17464, Abcam, Shanghai, China) followed by secondary antibody (HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L),

(18) Nuclear Receptor PXR Confers Irradiation Resistance by Promoting DNA Damage Response Through Stabilization of ATF3Frontiers in OncologyMarch 16, 2022Xiaxia Niu, Hongmei Cui, Xinsheng Gu, Ting Wu, Min Sun, Changlong Zhou, Mei Maanti-mouse IgG VeriBlot for IP secondary antibody (HRP) (ab131368) and anti-Phosphor-(Ser/Thr) Phe antibody (ab17464, 1:1000) were purchased from Abcam (Cambridge, MA, USA). FLAG (F3165, 1:4000) and actin (A5441, 1:5000)

(19) Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearanceEMBO reportsFebruary 3, 2022Chen Y, Wu X, Zhang J, Pan G, Wang X, Guo X, Wang J, Cui X, Gao H, Cheng M, et al.Vps35 (ab10099, RRID:AB_296841), transferrin receptor (TfR) (ab84036, RRID:AB_10673794), p‐(Ser/Thr) (ab17464, RRID:AB_443891), LDL receptor‐related protein 1 (LRP1) (ab92544, RRID:AB_2234877, for immunofluorescence),

(20) Systemic inhibition or global deletion of CaMKK2 protects against post-traumatic osteoarthritisOsteoarthritis and cartilageJanuary 1, 2022Mével E, Shutter JA, Ding X, Mattingly BT, Williams JN, Li Y, Huls A, Kambrath AV, Trippel SB, Wagner D, et al.anti-phospho CaMKIV (Thr196, Thr200)","Invitrogen, Waltham, MA",PA5–105011,1:1001:1000,IHCIB Rabbit anti-Phospho - (Ser/Thr) Phe antibody,Abcam,Ab17464,1:1000,IB mouse anti-CaMKK,BD,610544,1:1000,IB rabbit anti-p-AMPKa (Thr172),"Cell signaling, Danvers,

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Anti-Phospho - (Ser/Thr) Phe antibody from Abcam

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Anti-Phospho - (Ser/Thr) Phe antibody from Abcam

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