Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] ab18230 from Abcam

Product Specs
ItemAnti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab18230
Antibody TypePrimary
IsotypeIgG1
ApplicationsImmunocytochemistry, Immunocytochemistry/ Immunofluorescence
Quantity100 UG
ClonalityMonoclonal
CloneF-tag-01
HostMouse, Nude mouse
Conjugate/TagUnconjugated
Concentration1 mg/ml
ReactivitySpecies independent
TargetDDDDK tag

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Butyrate facilitates immune clearance of colorectal cancer cells by suppressing STAT1-mediated PD-L1 expressionClinics (Sao Paulo)November 5, 2023Yan Zhang, Yuan Tao, Yuqing Gu, Qiujie Macells were permeabilized for 10 min and blocked for 1h before incubation with anti-Flag antibodies (#ab18230; Abcam, USA) overnight. Next, the samples were washed and incubated with secondary antibodies for 1h.

(2) HERC2 promotes inflammation-driven cancer stemness and immune evasion in hepatocellular carcinoma by activating STAT3 pathwayJournal of experimental & clinical cancer research : CRFebruary 1, 2023Liu Y, Xu Q, Deng F, Zheng Z, Luo J, Wang P, Zhou J, Lu X, Zhang L, Chen Z, et al.cells were fixed and permeabilized. Next, the cells were incubated with an anti-DDDDK tag antibody (ab18230, Abcam) overnight at 4 °C. Nuclei were stained with DAPI for 30 min at 37 °C. To detect the interaction

(3) Nuclear accumulation of host transcripts during Zika Virus InfectionPLoS pathogensJanuary 1, 2023Leon KE, Khalid MM, Flynn RA, Fontaine KA, Nguyen TT, Kumar GR, Simoneau CR, Tomar S, Jimenez-Morales D, Dunlap M, et al.Abcam, Cambridge, UK, ab109363), anti-FREM2 (Invitrogen, Carlsbad, CA, PA5-20982), anti-FLAG (Abcam, ab18230), anti-Actin (CST, 4967S), anti-GFAP (Abcam, ab53554), anti-Nestin (Abcam, ab22035) and anti-Beta III tubulin

(4) Phosphorylation of PFKFB4 by PIM2 promotes anaerobic glycolysis and cell proliferation in endometriosisCell Death & DiseaseSeptember 15, 2022Chao Lu, Pengyun Qiao, Ruihai Fu, Yadi Wang, Jiayi Lu, Xi Ling, Lu Liu, Yujun Sun, Chune Ren, Zhenhai YuPFKFB4 was generated by Shanghai Genomic Inc. Anti-DDDDK tag Rabbit (ab205606, 1:3000), Flag tag Mouse (ab18230, 1:1000), HA Tag Mouse (ab1424, 1:2000), HA tag Rabbit (ab236632, 1:1000) were purchased from Abcam.

(5) Tumor-derived extracellular vesicles shuttle c-Myc to promote gastric cancer growth and metastasis via the KCNQ1OT1/miR-556-3p/CLIC1 axisCell Death & DiseaseMarch 8, 2022Bopei Li, Yeyang Chen, Liang Liang, Ye Wang, Weijia Huang, Kun Zhao, Siyu Liu, Guofei Deng, Junqiang Chentheir permeability. The cells were sealed for 1 h with 5% BSA and probed overnight with anti-FLAG (ab18230, Abcam) primary antibody on the shaking table. The primary antibody was discarded, followed by 1-h cell

(6) METTL14-mediated Lnc-LSG1 m6A modification inhibits clear cell renal cell carcinoma metastasis via regulating ESRP2 ubiquitinationMolecular therapy. Nucleic acidsMarch 8, 2022Shen D, Ding L, Lu Z, Wang R, Yu C, Wang H, Zheng Q, Wang X, Xu W, Yu H, et al.polyclonal, GTX31826, GeneTex; rabbit polyclonal, NBP2-13972, Novus), anti-Flag (mouse monoclonal, ab18230, Abcam), anti-Ubiquitin (rabbit monoclonal, ab134953, Abcam), anti-GAPDH (mouse monoclonal, ab8245,

(7) Zinc antagonizes iron-regulation of tyrosine hydroxylase activity and dopamine production in Drosophila melanogasterBMC BiologyDecember 1, 2021Guiran Xiao, Mengran Zhao, Zhihua Liu, Fan Du, Bing Zhou(anti-PDI, 1:500), and imaged with the ZEISS LSM780 microscope. Anti-FLAG mouse monoclonal antibody (ab18230), anti-FLAG rabbit polyclonal antibody (ab1162), anti-GM130 rabbit polyclonal antibody (ab30637), and

(8) Viromes and surveys of RNA viruses in camel-derived ticks revealing transmission patterns of novel tick-borne viral pathogens in KenyaEmerging Microbes & InfectionsOctober 17, 2021You Zhang et al.The protein expression in transfected cells was validated by western blot using anti-Flag antibody (AB18230, Abcam, Cambridge, UK) (data not shown). The light unit (LU) of each Ruc-viral antigen was measured

(9) Paeonol attenuates inflammation by confining HMGB1 to the nucleusJournal of Cellular and Molecular MedicineMarch 1, 2021Jifei Miao, Jun Zhong, Jiao Lan, Sen Ye, Peng Ye, Siyan Li, Aijia You, Xianjie Chen, Xiaoyi Liu, Hui LiTNF‐α (ab1793), NF‐κB P65 (ab16502), HMGB1 (ab18256), β‐actin (ab6276), Lamin B1 (ab16048) and Flag (ab18230) were bought from Abcam. Secondary antibodies targeting mouse antibodies (ab6789 and ab150113) or rabbit

(10) Paraspeckle Promotes Hepatocellular Carcinoma Immune Escape by Sequestering IFNGR1 mRNACellular and molecular gastroenterology and hepatologyJanuary 1, 2021Zan J, Zhao X, Deng X, Ding H, Wang B, Lu M, Wei Z, Huang Z, Wang S.STAT1 (pSTAT1) (1:1000, 9167; Cell Signal Technology), NONO (1:1000, ab70335; Abcam), flag (1:2000, ab18230; Abcam), Phospho-(Ser/Thr) (1:500, ab117253; Abcam), and Phospho-Tyrosine (P-Tyr) (1:500, 8954; Cell

(11) Overexpression of long non-coding RNA SBF2-AS1 promotes cell progression in esophageal squamous cell carcinoma (ESCC) by repressing miR-494 to up-regulate PFN2 expressionBiology OpenMarch 30, 2020Qiu Zhang, Xixiang Pan, Dongyang Youantibodies against PFN2 (1:1000 dilution, ab230673, Abcam, Cambridge, MA, USA), N-cadherin (1:1000 dilution, ab18230, Abcam), Vimentin (1:1000 dilution, ab18230, Abcam) and E-cadherin (1:1000 dilution, ab76055, Abcam)

(12) AQP1 suppression by ATF4 triggers trabecular meshwork tissue remodelling in ET‐1‐induced POAGJournal of Cellular and Molecular MedicineMarch 1, 2020Yingying Zhao, Huazhang Zhu, Yangfan Yang, Yiming Ye, Youli Yao, Xiaoyan Huang, Yixiang Zhang, Xingsheng Shu, Xianxiong Chen, Yatao Yang, et al.(Ser51),Rabbit,Cell singal technology,3398 GAPDH,Rabbit,Cell singal technology,5174 Flag,Mouse,Abcam,ab18230

(13) Therapeutic targeting of circ-CUX1/EWSR1/MAZ axis inhibits glycolysis and neuroblastoma progressionEMBO molecular medicineDecember 1, 2019Li H, Yang F, Hu A, Wang X, Fang E, Chen Y, Li D, Song H, Wang J, Guo Y, et al.(ab60720), S100A9 (ab92507), KLF10 (ab73537), TXNIP (ab188865), AMPKβ1 (ab32112), β‐actin (ab8227), Flag (ab18230), Myc (ab9106, Abcam Inc., Cambridge, MA), GST (sc‐33614), or histone H3 (sc‐10809, Santa Cruz Biotechnology). Gene

(14) TRIM47 is up-regulated in colorectal cancer, promoting ubiquitination and degradation of SMAD4Journal of Experimental & Clinical Cancer ResearchDecember 1, 2019Qian Liang, Chaotao Tang, Mingyu Tang, Qingwei Zhang, Yunjie Gao, Zhizheng Ge(1:1000, CST, USA, 13667), rabbit anti-CDH1 (1:1000, CST, USA, 3195), mouse anti-Flag (1:1000, Abcam, USA, ab18230), GAPDH (1:3000; KangChen, Shanghai, China, KC-5G5). Immunohistochemistry The tissue microarray sections

(15) The β-catenin/YAP signaling axis is a key regulator of melanoma-associated fibroblastsSignal transduction and targeted therapyJanuary 1, 2019Liu T, Zhou L, Yang K, Iwasawa K, Kadekaro AL, Takebe T, Andl T, Zhang Y.(Cell signaling, 14074, 1:200), rabbit anti-K14 (Biolegend, PRB-155P 1:1000), and rabbit anti-N-cadherin (Abcam, ab18230, 1:200), and they each were diluted in Duolink antibody diluent. Diluted primary antibodies were then

(16) Expression and in vitro assessment of tumorigenicity for NOD1 and NOD2 receptors in breast cancer cell linesBMC Research NotesDecember 1, 2018Fernando J. Velloso, Mari Cleide Sogayar, Ricardo G. CorreaWestern blotting Ectopic proteins were detected by immunoblotting using mouse monoclonal anti-FLAG antibody (ab18230, Abcam). Reversible Ponceau staining was used to control the equal loading of protein lysates. Growth

(17) HR38, an ortholog of NR4A family nuclear receptors, mediates 20-hydroxyecdysone regulation of carbohydrate metabolism during mosquito reproductionInsect Biochemistry and Molecular BiologyMay 1, 2018Dujuan Dong, Yang Zhang, Vlastimil Smykal, Lin Ling, Alexander S. Raikhelantibodies were used: anti-His antiserum (Abcam, ab18184) for AHR38-His; anti-Flag antiserum (Abcam, ab18230) for EcR-Flag and USP-Flag; β-actin monoclonal antibody (Sigma, A5441) for actin. 2.7. In vitro fat

(18) EFA6 regulates selective polarised transport and axon regeneration from the axon initial segmentJournal of cell scienceNovember 1, 2017Eva R, Koseki H, Kanamarlapudi V, Fawcett JW.1:50; rabbit anti-ARF6 ab77581, Abcam, 1:100; rabbit anti-GST ab19256, Abcam, 1:400; mouse anti-FLAG ab18230, Abcam, 1:750; mouse anti-β-actin ab8226, Abcam, 1:1000; rabbit anti-tRFP ab233, Evrogen, 1:1000; anti-integrin

(19) Yin Yang 1 Dynamically Regulates Antiviral Innate Immune Responses During Viral InfectionCellular Physiology and BiochemistryJuly 25, 2017Jie Zan, Hao Zhang, Ai-Ping Gu, Kai-Lun Zhong, Min-Yi Lu, Xiao-Xin Bai, Jin-Yang Zhang, Jun CaiTechnology), STAT2 (72604, Cell Signal Technology), phosphorylated STAT2 (pSTAT2) (ab53132, Abcam), flag (ab18230, Abcam) and Myc (ab18185, Abcam) were used. Co-immunoprecipitation was performed as previously described

(20) The aspirin-induced long non-coding RNA OLA1P2 blocks phosphorylated STAT3 homodimer formationGenome BiologyDecember 1, 2016Haiyan Guo, Jun Liu, Qiwen Ben, Yuehong Qu, Man Li, Ying Wang, Wantao Chen, Jianjun ZhangSanta Cruz, Dallas, TX, USA), Flag antibody (ab49969, 1:3,000, Abcam, Cambridge, MA, USA) or HA antibody (ab18230, 1:3,000, Abcam, Cambridge, MA, USA) in 5 % BSA overnight at 4 °C. Secondary antibodies (1:3,000) were

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Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] from Abcam

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Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] from Abcam

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