Fig 1: Intra- and inter-individual variability in protein expression in response to ionizing radiationCells were exposed to sham dose or 4 Gy of X-ray and collected for western blot analysis 2 h after exposure.(A) Western blot analysis of 5 different cell types isolated from the same neonatal donor; K – keratinocytes, M – melanocytes, F –Fibroblasts, EC – microvascular endothelial cells, A – preadipocytes.(B) Western blot analysis of 6 different donors of neonatal keratinocytes; donor F is the same one as in (A). The following antibodies were used for western blotting: Ataxia Telangiectasia Mutated (ATM), Abcam (ab32420, dilution 1:5,000); pATM ser 1981, Abcam (ab81292, dilution 1:5,000); Tumor Protein P53 (TP53), Santa Cruz (sc-126, dilution 1:10,000); Cyclin Dependent Kinase Inhibitor 1A (CDKN1A), Cell Signalling (2947, dilution 1:30,000); Lamin B1 (LMNB1), Abcam (ab16048, dilution 1:5,000); Tubulin Alpha 1b (TUBA1B), Proteintech (66031-1-AP, dilution 1:100,000); Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), Proteintech (60004-1-Ig, dilution 1:100,000); Actin beta (ACTB), Proteintech (20536-1-AP, dilution 1:5,000).