Fig 1: Differential proteomic profiles of Olfm4-H and Olfm4-L neutrophil subsets in healthy blood donors. Olfm4-H and Olfm4-L neutrophils isolated from healthy blood donors (n = 8) were sorted by FACS and analysed by LC-MS/MS. a Volcano plot showing median log2 abundance ratios between the identified proteins in Olfm4-H and Olfm4-L neutrophils and their p values. b Left box-and-whisker plot showing proteins with a statistically significant positive median log2 abundance ratio, indicating increased abundance in the Olfm4-H neutrophils. Right box-and-whisker plot showing proteins with a statistically significant negative median log2 abundance ratio, indicating increased abundance in the Olfm4-L neutrophils. c Neutrophils from healthy blood donors (n = 7) were isolated and stained for proteins of interest and analysed by live imaging flow cytometry. Box-and-whisker plot comparing median fluorescence intensity of the immunofluorescently stained proteins Rab3d, S100A7, Rab3a, and CXCR1 between Olfm4-H and Olfm4-L neutrophils. Wilcoxon signed rank tests were used to test for statistical significance. Common contaminants and histones are not shown.