Fig 1: FAM46C overexpression resulted in G2/M phase arrest (A) and a significant increase of cell apoptosis (B) as determined by flow cytometry analyses at 48 h after viral infection. ***P < 0.001 as compared with Vector virus-infected cells. (C) Protein levels of FAM46C and cell growth-related factors (PCNA, Cyclin B1 and CDK1) were evaluated by Western blotting. (D) Protein levels of FAM46C and apoptotic factors (Bcl-2, Bax and XIAP) were assessed. (E) The levels of Ras, p-MEK1/2, MEK, p-ERK1/2 and ERK1/2 were evaluated. All experiments shown were performed independently at least three times. **P < 0.01 and ***P < 0.001 as compared with Vector virus-infected cells.