Fig 2: A single cell RNA-seq (scRNA-seq) of human degenerative nucleus pulposus (NP). (A) Schematic workflow of the experimental strategy. (B) Sixteen clusters (0-15) of human degenerative NP were identified by specifical genes into five cell clusters and shown by uniform manifold approximation and projection (UMAP). (C) Feature plot showing differentially expressed genes in five clusters. (D) Ridge plot showing differentially expressed genes in five clusters. (E) Heatmap showing differentially expressed genes in five clusters. (F) Representative immunohistochemistry staining of IL1B, COL2A1, COL4A1, LGALS1, COL1A1, for the five cell clusters of degenerative human NP. Scale bar, 100 μm. n=6. (G) UMAP indicating the heterogeneity of cell cluster percentage in four samples.