Fig 2: ILK expression in different cell types in colon cancer. ILK expression was examined in patient colon cancers via the immune cell atlas platform [Single Cell Portal (broadinstitute.org)]. (A) t-distributed stochastic neighbor embedding (t-SNE) plot showing B cells (B), epithelial cancer and non-cancer cells (Epi), myeloid cells (Myeloid), plasma cells (Plasma), and T and NK and innate lymphoid cells (TNKILC) of colon cancer. (B) ILK expression in all cell clusters. (C) FOXP3 expression for Tregs in all cell clusters. (D) CD163 expression for M2 macrophages in all cell clusters.

Fig 3: ILK expression is negatively correlated with tumor purity and positively correlated with immune cell infiltration in COAD, STAD and LUSC. The correlations between ILK gene expression and tumor purity are on the left panel. The correlation between ILK and immune cells including B cells, CD8+ T cells, CD4+ T cells, macrophages, neutrophils and DCs are corrected by tumor purity (partial.cor, partial Spearman’s correlation rho). (upper row) COAD; (second row) KIRC; (third row) LUSC; (lower row) STAD. Significant P-value is < 0.05.

Fig 4: ILKR211A induces Hsp70 expression in the infarct zone of LAD-ligated mice.Western blot analysis of the protein levels of ILK, Hsp70 and Hsp90 in the lysates of myocardial infarct zone (MI) and control, viable, non infarcted myocardial zone (C) obtained from the hearts of two 28 days post LAD ligated TgR211A mice (+) and their littermate controls (-). GAPDH served as a loading control.

Fig 5: ILK Induces Hsc70 and Hsp70 Expression in Human Cardiomyocytes. A, (top panels) Phase contrast and fluorescent microscopy images of human IPSC-derived cardiomyocytes (Hu iPS-CMs) (on the left) and primary rabbit cardiomyocytes (on the right) transduced with GFP-linked vector (ad-GFP) versus non transduced cells (C). The Hu iPS-CM cells express monomeric red fluorescent protein and blasticidin resistance under the control of the α-myosin heavy chain promoter that allows simultaneous cardiomyocyte purification and identification. Green fluorescence indicates efficient transduction of ILK constructs. Scale bar, 80 µm. (bottom panels) Western blots showing expression levels of ILK, Hsp70 and Hsc70 in Hu iPS-CMs (on the left) and in primary rabbit cardiomyocytes (on the right) transduced with ILKWT and ILKR211A as compared to non-transduced cells and cells transduced with the vector bearing GFP only. GAPDH was used as a loading control. B, Immunoblot for ILK expression levels in Hu IPS-CMs transduced with ad-ILKWT and ad-ILKR211A as compared to non-transduced cells following treatment with cycloheximide (CHX) at 80 µM for 0, 3, 6 and 18 hours. C, Western blot analysis showing the protein levels of ILK, Hsp70 and Hsp-90 in Hu iPS-CMs transduced with ad-GFP, ad-ILKWT, ad-ILKR211A and in non-transduced cells (C) following treatment with indicated doses of Hsc/p70-ATPase inhibitor (Ver-155008) for 24 hours. GAPDH was used as a loading control. Each experiment was performed at least three times on independent samples and one representative blot is shown.